Urinary Metabolic Profiles After Vitamin D Versus Vitamin D Supplementation in Prediabetes

Overview

Journal J Clin Transl Endocrinol

Specialty Endocrinology

Date 2019 Jun 14

PMID 31193444

Citations 3

Authors

Laor Chailurkit

Hataikarn Nimitphong

Sunee Saetung

Boonsong Ongphiphadhanakul

Affiliations

Soon will be listed here.

Abstract

Aim: To assess the potential biological differences between vitamin D and D using urinary metabolite profiles in response to vitamin D or D supplementation.

Method: Subjects consisted of 29 subjects with impaired fasting glucose and/or impaired glucose tolerance. Subjects were randomized into two groups, vitamin D (20,000 IU weekly, n = 14) or vitamin D (15,000 IU weekly, n = 15). Urine and serum samples were taken at two different time points for each subject (at baseline and at 12 weeks). Urinary metabolite profiling was performed by liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS). Serum calcium was analyzed on an automated biochemical analyzer and serum intact parathyroid hormone was determined by electrochemiluminescence immunoassay.

Results: At baseline, there was no statistically significant difference in clinical characteristics including age, gender, body mass index, waist circumference and 25-hydroxyvitamin D (25(OH)D) levels between the 2 groups. Weekly administration of 20,000 U D for 12 weeks resulted in comparable 25(OH)D concentrations as compared to weekly 15,000 U D supplementation (97.8 ± 305 vs. 96.8 ± 3.4 nmol/L,  = 0.84). No difference in serum calcium (2.3 ± 0.03 vs. 2.2 ± 0.03 nmol/L,  = 0.52) or intact parathyroid hormone (5.3 ± 0.3 vs. 4.9 ± 0.5 pmol/L,  = 0.54) at 12 weeks was found. Principle component analysis did not reveal apparent segregation of metabolites according to D or D supplementation. Moreover, using partial least square regression, no apparent separation between the D and the D group was found. No important metabolite influencing the separation of the D from the D group was found using variables importance on projection analysis.

Conclusions: At comparable circulating 25(OH)D concentrations, vitamin D or D supplementation does not appear to result in different urinary metabolite profiles. Our finding does not support a biological difference between vitamin D and D.

Citing Articles

Standard Doses of Cholecalciferol Reduce Glucose and Increase Glutamine in Obesity-Related Hypertension: Results of a Randomized Trial.

Santos C, Carvalho R, Fonseca A, Branco M, Alves M, Jarak I Int J Mol Sci. 2024; 25(6).

PMID: 38542390 PMC: 10969945. DOI: 10.3390/ijms25063416.

Applications of Metabolomics in Calcium Metabolism Disorders in Humans.

Podgorska B, Wielogorska-Partyka M, Godzien J, Sieminska J, Ciborowski M, Szelachowska M Int J Mol Sci. 2022; 23(18).

PMID: 36142318 PMC: 9499180. DOI: 10.3390/ijms231810407.

Vitamin D supplementation is associated with serum uric acid concentration in patients with prediabetes and hyperuricemia.

Nimitphong H, Saetung S, Chailurkit L, Chanprasertyothin S, Ongphiphadhanakul B J Clin Transl Endocrinol. 2021; 24:100255.

PMID: 33898272 PMC: 8054183. DOI: 10.1016/j.jcte.2021.100255.

References

Nicholson J, Lindon J, Holmes E . 'Metabonomics': understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data. Xenobiotica. 1999; 29(11):1181-9. DOI: 10.1080/004982599238047. View

Vieth R, Chan P, MacFarlane G . Efficacy and safety of vitamin D3 intake exceeding the lowest observed adverse effect level. Am J Clin Nutr. 2001; 73(2):288-94. DOI: 10.1093/ajcn/73.2.288. View

Piaseu N, Komindr S, Chailurkit L, Ongphiphadhanakul B, Chansirikarn S, Rajatanavin R . Differences in bone mineral density and lifestyle factors of postmenopausal women living in Bangkok and other provinces. J Med Assoc Thai. 2001; 84(6):772-81. View

Smith C, OMaille G, Want E, Qin C, Trauger S, Brandon T . METLIN: a metabolite mass spectral database. Ther Drug Monit. 2006; 27(6):747-51. DOI: 10.1097/01.ftd.0000179845.53213.39. View

Romagnoli E, Mascia M, Cipriani C, Fassino V, Mazzei F, DErasmo E . Short and long-term variations in serum calciotropic hormones after a single very large dose of ergocalciferol (vitamin D2) or cholecalciferol (vitamin D3) in the elderly. J Clin Endocrinol Metab. 2008; 93(8):3015-20. DOI: 10.1210/jc.2008-0350. View

Chailurkit L, Kruavit A, Rajatanavin R . Vitamin D status and bone health in healthy Thai elderly women. Nutrition. 2010; 27(2):160-4. DOI: 10.1016/j.nut.2009.12.001. View

Elnenaei M, Chandra R, Mangion T, Moniz C . Genomic and metabolomic patterns segregate with responses to calcium and vitamin D supplementation. Br J Nutr. 2010; 105(1):71-9. DOI: 10.1017/S0007114510003065. View

Bouvard B, Annweiler C, Salle A, Beauchet O, Chappard D, Audran M . Extraskeletal effects of vitamin D: facts, uncertainties, and controversies. Joint Bone Spine. 2010; 78(1):10-6. DOI: 10.1016/j.jbspin.2010.10.011. View

Heaney R, Recker R, Grote J, Horst R, Armas L . Vitamin D(3) is more potent than vitamin D(2) in humans. J Clin Endocrinol Metab. 2010; 96(3):E447-52. DOI: 10.1210/jc.2010-2230. View

10.

OSullivan A, Gibney M, Connor A, Mion B, Kaluskar S, Cashman K . Biochemical and metabolomic phenotyping in the identification of a vitamin D responsive metabotype for markers of the metabolic syndrome. Mol Nutr Food Res. 2011; 55(5):679-90. DOI: 10.1002/mnfr.201000458. View

11.

Gupta A, Brashear M, Johnson W . Prediabetes and prehypertension in healthy adults are associated with low vitamin D levels. Diabetes Care. 2011; 34(3):658-60. PMC: 3041202. DOI: 10.2337/dc10-1829. View

12.

Binkley N, Gemar D, Engelke J, Gangnon R, Ramamurthy R, Krueger D . Evaluation of ergocalciferol or cholecalciferol dosing, 1,600 IU daily or 50,000 IU monthly in older adults. J Clin Endocrinol Metab. 2011; 96(4):981-8. PMC: 3417158. DOI: 10.1210/jc.2010-0015. View

13.

Shankar A, Sabanayagam C, Kalidindi S . Serum 25-hydroxyvitamin d levels and prediabetes among subjects free of diabetes. Diabetes Care. 2011; 34(5):1114-9. PMC: 3114501. DOI: 10.2337/dc10-1203. View

14.

Pittas A, Nelson J, Mitri J, Hillmann W, Garganta C, Nathan D . Plasma 25-hydroxyvitamin D and progression to diabetes in patients at risk for diabetes: an ancillary analysis in the Diabetes Prevention Program. Diabetes Care. 2012; 35(3):565-73. PMC: 3322702. DOI: 10.2337/dc11-1795. View

15.

Deleskog A, Hilding A, Brismar K, Hamsten A, Efendic S, Ostenson C . Low serum 25-hydroxyvitamin D level predicts progression to type 2 diabetes in individuals with prediabetes but not with normal glucose tolerance. Diabetologia. 2012; 55(6):1668-78. DOI: 10.1007/s00125-012-2529-x. View

16.

Tripkovic L, Lambert H, Hart K, Smith C, Bucca G, Penson S . Comparison of vitamin D2 and vitamin D3 supplementation in raising serum 25-hydroxyvitamin D status: a systematic review and meta-analysis. Am J Clin Nutr. 2012; 95(6):1357-64. PMC: 3349454. DOI: 10.3945/ajcn.111.031070. View

17.

Pludowski P, Holick M, Pilz S, Wagner C, Hollis B, Grant W . Vitamin D effects on musculoskeletal health, immunity, autoimmunity, cardiovascular disease, cancer, fertility, pregnancy, dementia and mortality-a review of recent evidence. Autoimmun Rev. 2013; 12(10):976-89. DOI: 10.1016/j.autrev.2013.02.004. View

18.

Rajatanavin R, Chailurkit L, Saetung S, Thakkinstian A, Nimitphong H . The efficacy of calcium supplementation alone in elderly Thai women over a 2-year period: a randomized controlled trial. Osteoporos Int. 2013; 24(11):2871-7. DOI: 10.1007/s00198-013-2387-5. View

19.

Lehmann U, Hirche F, Stangl G, Hinz K, Westphal S, Dierkes J . Bioavailability of vitamin D(2) and D(3) in healthy volunteers, a randomized placebo-controlled trial. J Clin Endocrinol Metab. 2013; 98(11):4339-45. DOI: 10.1210/jc.2012-4287. View

20.

Finkelstein J, Pressman E, Cooper E, Kent T, Bar H, OBrien K . Vitamin D Status Affects Serum Metabolomic Profiles in Pregnant Adolescents. Reprod Sci. 2014; 22(6):685-95. PMC: 4502797. DOI: 10.1177/1933719114556477. View