MiR-22 Enhances the Radiosensitivity of Small-cell Lung Cancer by Targeting the WRNIP1

Overview

Journal J Cell Biochem

Specialties Biochemistry
Cell Biology

Date 2019 Jun 14

PMID 31190355

Citations 27

Authors

Wenhua Jiang

Xuemei Han

Jingrui Wang

Lin Wang

Zanmei Xu

Qiao Wei

Wenpan Zhang

Haitao Wang

Affiliations

Soon will be listed here.

Abstract

Small-cell lung cancer (SCLC) is an aggressive malignancy characterized by high cellular proliferation and early distant metastasis. Our study aimed to explore the effect of miR-22-3p (miR-22, for short) on SCLC radiosensitivity and its molecular mechanisms. The expression level of miR-22 was evaluated in a human normal lung epithelial cell line and a human SCLC cell line, and cell apoptosis and migration were detected. The expression of the miR-22 direct target WRNIP1 mRNA and protein were explored. Five differentially expressed genes were detected. The miR-22 expression in NCI-H446 was significantly decreased, and miR-22 overexpression significantly promoted cell apoptosis. miR-22 overexpression could significantly inhibit the cell migration of SCLC cells, and miR-22 had a negative regulatory effect on WRNIP1 mRNA and protein levels. KLK8 was downregulated, and the messenger RNA (mRNA) of four other genes (PC, SCUBE1, STC1, and GPM6A) was upregulated mRNA in cells overexpressing miR-22, which was in accordance with the bioinformatics analysis. miR-22 could enhance the radiosensitivity of SCLC by targeting WRNIP1.

Citing Articles

Mechanisms of GPM6A in Malignant Tumors.

Huang B, Li X Cancer Rep (Hoboken). 2025; 8(2):e70137.

PMID: 39957375 PMC: 11831008. DOI: 10.1002/cnr2.70137.

Targeting STK26 and ATG4B: miR-22-3p as a modulator of autophagy and tumor progression in HCC.

Li K, Bai Y, Wang J, Ren L, Mo A, Liu R Transl Oncol. 2024; 51:102214.

PMID: 39608212 PMC: 11635773. DOI: 10.1016/j.tranon.2024.102214.

Research Trends and Evolution in Radiogenomics (2005-2023): Bibliometric Analysis.

Wang M, Peng Y, Wang Y, Luo D Interact J Med Res. 2024; 13:e51347.

PMID: 38980713 PMC: 11267093. DOI: 10.2196/51347.

An innovative prognostic auxiliary for colon adenocarcinoma based on zinc finger protein genes.

Xu F, Sun J, Gu X, Zhou Q Transl Cancer Res. 2024; 13(4):1623-1641.

PMID: 38737696 PMC: 11082816. DOI: 10.21037/tcr-23-2158.

Evaluating CD133 as a Radiotheranostic Target in Small-Cell Lung Cancer.

Sarrett S, Rodriguez C, Delaney S, Hosny M, Sebastiano J, Santos-Coquillat A Mol Pharm. 2024; 21(3):1402-1413.

PMID: 38331430 PMC: 10915790. DOI: 10.1021/acs.molpharmaceut.3c01063.

References

Zhou J, Liu J, Cheng C, Patel T, Weller C, Piepmeier J . Biodegradable poly(amine-co-ester) terpolymers for targeted gene delivery. Nat Mater. 2011; 11(1):82-90. PMC: 4180913. DOI: 10.1038/nmat3187. View

Chun-Zhi Z, Lei H, An-Ling Z, Yan-Chao F, Xiao Y, Guang-Xiu W . MicroRNA-221 and microRNA-222 regulate gastric carcinoma cell proliferation and radioresistance by targeting PTEN. BMC Cancer. 2010; 10:367. PMC: 2914702. DOI: 10.1186/1471-2407-10-367. View

Johnson S, Grosshans H, Shingara J, Byrom M, Jarvis R, Cheng A . RAS is regulated by the let-7 microRNA family. Cell. 2005; 120(5):635-47. DOI: 10.1016/j.cell.2005.01.014. View

Kasinski A, Slack F . Epigenetics and genetics. MicroRNAs en route to the clinic: progress in validating and targeting microRNAs for cancer therapy. Nat Rev Cancer. 2011; 11(12):849-64. PMC: 4314215. DOI: 10.1038/nrc3166. View

Babar I, Cheng C, Booth C, Liang X, Weidhaas J, Saltzman W . Nanoparticle-based therapy in an in vivo microRNA-155 (miR-155)-dependent mouse model of lymphoma. Proc Natl Acad Sci U S A. 2012; 109(26):E1695-704. PMC: 3387084. DOI: 10.1073/pnas.1201516109. View

Shi Y, Zhang X, Tang X, Wang P, Wang H, Wang Y . MiR-21 is continually elevated long-term in the brain after exposure to ionizing radiation. Radiat Res. 2011; 177(1):124-8. DOI: 10.1667/rr2764.1. View

Tokuda E, Horimoto Y, Arakawa A, Himuro T, Senuma K, Nakai K . Differences in Ki67 expressions between pre- and post-neoadjuvant chemotherapy specimens might predict early recurrence of breast cancer. Hum Pathol. 2017; 63:40-45. DOI: 10.1016/j.humpath.2017.02.005. View

Babar I, Czochor J, Steinmetz A, Weidhaas J, Glazer P, Slack F . Inhibition of hypoxia-induced miR-155 radiosensitizes hypoxic lung cancer cells. Cancer Biol Ther. 2011; 12(10):908-14. PMC: 3280906. DOI: 10.4161/cbt.12.10.17681. View

Kozomara A, Griffiths-Jones S . miRBase: integrating microRNA annotation and deep-sequencing data. Nucleic Acids Res. 2010; 39(Database issue):D152-7. PMC: 3013655. DOI: 10.1093/nar/gkq1027. View

10.

Du Y, Gu X, Cheng S, Li L, Liu H, Hu L . The oncogenetic role of stanniocalcin 1 in lung adenocarcinoma: a promising serum candidate biomarker for tracking lung adenocarcinoma progression. Tumour Biol. 2015; 37(4):5633-44. DOI: 10.1007/s13277-015-4431-x. View

11.

Yoshimura A, Seki M, Enomoto T . The role of WRNIP1 in genome maintenance. Cell Cycle. 2017; 16(6):515-521. PMC: 5384577. DOI: 10.1080/15384101.2017.1282585. View

12.

Gu X, Fu C, Lin L, Liu S, Su X, Li A . miR-124 and miR-9 mediated downregulation of HDAC5 promotes neurite development through activating MEF2C-GPM6A pathway. J Cell Physiol. 2017; 233(1):673-687. DOI: 10.1002/jcp.25927. View

13.

Lee S, Jeon H, Ju M, Kim C, Yoon G, Han S . Wnt/Snail signaling regulates cytochrome C oxidase and glucose metabolism. Cancer Res. 2012; 72(14):3607-17. DOI: 10.1158/0008-5472.CAN-12-0006. View

14.

Jiang W, Han X, Wang J, Wang L, Xu Z, Wei Q . miR-22 enhances the radiosensitivity of small-cell lung cancer by targeting the WRNIP1. J Cell Biochem. 2019; 120(10):17650-17661. PMC: 6771739. DOI: 10.1002/jcb.29032. View

15.

Girardi C, De Pitta C, Casara S, Sales G, Lanfranchi G, Celotti L . Analysis of miRNA and mRNA expression profiles highlights alterations in ionizing radiation response of human lymphocytes under modeled microgravity. PLoS One. 2012; 7(2):e31293. PMC: 3276573. DOI: 10.1371/journal.pone.0031293. View

16.

Hermeking H . MicroRNAs in the p53 network: micromanagement of tumour suppression. Nat Rev Cancer. 2012; 12(9):613-26. DOI: 10.1038/nrc3318. View

17.

Xin M, Qiao Z, Li J, Liu J, Song S, Zhao X . miR-22 inhibits tumor growth and metastasis by targeting ATP citrate lyase: evidence in osteosarcoma, prostate cancer, cervical cancer and lung cancer. Oncotarget. 2016; 7(28):44252-44265. PMC: 5190093. DOI: 10.18632/oncotarget.10020. View

18.

Leuzzi G, Marabitti V, Pichierri P, Franchitto A . WRNIP1 protects stalled forks from degradation and promotes fork restart after replication stress. EMBO J. 2016; 35(13):1437-51. PMC: 4931187. DOI: 10.15252/embj.201593265. View

19.

Phannasil P, Thuwajit C, Warnnissorn M, Wallace J, MacDonald M, Jitrapakdee S . Pyruvate Carboxylase Is Up-Regulated in Breast Cancer and Essential to Support Growth and Invasion of MDA-MB-231 Cells. PLoS One. 2015; 10(6):e0129848. PMC: 4467472. DOI: 10.1371/journal.pone.0129848. View

20.

He L, Hannon G . MicroRNAs: small RNAs with a big role in gene regulation. Nat Rev Genet. 2004; 5(7):522-31. DOI: 10.1038/nrg1379. View