YshB Promotes Intracellular Replication and Is Required for Virulence

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2019 Jun 12

PMID 31182500

Citations 6

Authors

Rajdeep Bomjan

Mei Zhang

Daoguo Zhou

Affiliations

Soon will be listed here.

Abstract

virulence requires the initial invasion of host cells, followed by modulation of the intracellular environment for survival and replication. In an effort to characterize the role of small RNAs in pathogenesis, we inadvertently identified a 5-kDa protein named YshB that is involved in the intracellular survival of We show here that expression is upregulated upon entry into macrophages. When expression is upregulated before bacterial entry, invasion efficiency is inhibited. Lack of YshB resulted in reduced bacterial survival within the macrophages and led to reduced virulence in a mouse model of infection. gastroenteritis is one of the most common causes of foodborne disease, possibly affecting millions of people globally each year. Here we characterize the role of a novel small protein, YshB, in mediating intracellular survival. This elucidation adds to the body of knowledge regarding how this bacterium achieves intracellular survival.

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References

Hersh D, Monack D, Smith M, Ghori N, Falkow S, Zychlinsky A . The Salmonella invasin SipB induces macrophage apoptosis by binding to caspase-1. Proc Natl Acad Sci U S A. 1999; 96(5):2396-401. PMC: 26795. DOI: 10.1073/pnas.96.5.2396. View

Deiwick J, Nikolaus T, Erdogan S, Hensel M . Environmental regulation of Salmonella pathogenicity island 2 gene expression. Mol Microbiol. 1999; 31(6):1759-73. DOI: 10.1046/j.1365-2958.1999.01312.x. View

Rakeman J, Bonifield H, MILLER S . A HilA-independent pathway to Salmonella typhimurium invasion gene transcription. J Bacteriol. 1999; 181(10):3096-104. PMC: 93764. DOI: 10.1128/JB.181.10.3096-3104.1999. View

Lucas R, Lostroh C, DiRusso C, Spector M, Wanner B, Lee C . Multiple factors independently regulate hilA and invasion gene expression in Salmonella enterica serovar typhimurium. J Bacteriol. 2000; 182(7):1872-82. PMC: 101869. DOI: 10.1128/JB.182.7.1872-1882.2000. View

Datsenko K, Wanner B . One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A. 2000; 97(12):6640-5. PMC: 18686. DOI: 10.1073/pnas.120163297. View

Hensel M . Salmonella pathogenicity island 2. Mol Microbiol. 2000; 36(5):1015-23. DOI: 10.1046/j.1365-2958.2000.01935.x. View

Lucas R, Lee C . Unravelling the mysteries of virulence gene regulation in Salmonella typhimurium. Mol Microbiol. 2000; 36(5):1024-33. DOI: 10.1046/j.1365-2958.2000.01961.x. View

Galan J, Zhou D . Striking a balance: modulation of the actin cytoskeleton by Salmonella. Proc Natl Acad Sci U S A. 2000; 97(16):8754-61. PMC: 34008. DOI: 10.1073/pnas.97.16.8754. View

Prouty A, Gunn J . Salmonella enterica serovar typhimurium invasion is repressed in the presence of bile. Infect Immun. 2000; 68(12):6763-9. PMC: 97778. DOI: 10.1128/IAI.68.12.6763-6769.2000. View

10.

Nishino K, Yamaguchi A . Overexpression of the response regulator evgA of the two-component signal transduction system modulates multidrug resistance conferred by multidrug resistance transporters. J Bacteriol. 2001; 183(4):1455-8. PMC: 95021. DOI: 10.1128/JB.183.4.1455-1458.2001. View

11.

Pessi G, Williams F, Hindle Z, Heurlier K, Holden M, Camara M . The global posttranscriptional regulator RsmA modulates production of virulence determinants and N-acylhomoserine lactones in Pseudomonas aeruginosa. J Bacteriol. 2001; 183(22):6676-83. PMC: 95500. DOI: 10.1128/JB.183.22.6676-6683.2001. View

12.

Galan J . Salmonella interactions with host cells: type III secretion at work. Annu Rev Cell Dev Biol. 2001; 17:53-86. DOI: 10.1146/annurev.cellbio.17.1.53. View

13.

Fettes P, Forsbach-Birk V, Lynch D, Marre R . Overexpresssion of a Legionella pneumophila homologue of the E. coli regulator csrA affects cell size, flagellation, and pigmentation. Int J Med Microbiol. 2001; 291(5):353-60. DOI: 10.1078/1438-4221-00141. View

14.

Takaya A, Tomoyasu T, Tokumitsu A, Morioka M, Yamamoto T . The ATP-dependent lon protease of Salmonella enterica serovar Typhimurium regulates invasion and expression of genes carried on Salmonella pathogenicity island 1. J Bacteriol. 2001; 184(1):224-32. PMC: 134781. DOI: 10.1128/JB.184.1.224-232.2002. View

15.

Uzzau S, Rubino S, Bossi L . Epitope tagging of chromosomal genes in Salmonella. Proc Natl Acad Sci U S A. 2001; 98(26):15264-9. PMC: 65018. DOI: 10.1073/pnas.261348198. View

16.

Beuzon C, Holden D . Use of mixed infections with Salmonella strains to study virulence genes and their interactions in vivo. Microbes Infect. 2002; 3(14-15):1345-52. DOI: 10.1016/s1286-4579(01)01496-4. View

17.

Ren D, Sims J, Wood T . Inhibition of biofilm formation and swarming of Escherichia coli by (5Z)-4-bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone. Environ Microbiol. 2002; 3(11):731-6. DOI: 10.1046/j.1462-2920.2001.00249.x. View

18.

Akbar S, Schechter L, Lostroh C, Lee C . AraC/XylS family members, HilD and HilC, directly activate virulence gene expression independently of HilA in Salmonella typhimurium. Mol Microbiol. 2003; 47(3):715-28. DOI: 10.1046/j.1365-2958.2003.03322.x. View

19.

Takaya A, Suzuki M, Matsui H, Tomoyasu T, Sashinami H, Nakane A . Lon, a stress-induced ATP-dependent protease, is critically important for systemic Salmonella enterica serovar typhimurium infection of mice. Infect Immun. 2003; 71(2):690-6. PMC: 145356. DOI: 10.1128/IAI.71.2.690-696.2003. View

20.

Weilbacher T, Suzuki K, Dubey A, Wang X, Gudapaty S, Morozov I . A novel sRNA component of the carbon storage regulatory system of Escherichia coli. Mol Microbiol. 2003; 48(3):657-70. DOI: 10.1046/j.1365-2958.2003.03459.x. View