Plasma MicroRNA MiR-26b As a Potential Diagnostic Biomarker of Degenerative Myelopathy in Pembroke Welsh Corgis

Overview

Journal BMC Vet Res

Publisher Biomed Central

Specialty Veterinary Medicine

Date 2019 Jun 12

PMID 31182094

Citations 7

Authors

Kohei Nakata

Kazuki Heishima

Hiroki Sakai

Osamu Yamato

Yu Furusawa

Hidetaka Nishida

Sadatoshi Maeda

Hiroaki Kamishina

Affiliations

Soon will be listed here.

Abstract

Background: Degenerative myelopathy (DM) is a progressive neurodegenerative disease frequently found in Pembroke Welsh Corgis (PWCs). Most DM-affected PWCs are homozygous for the mutant superoxide dismutase 1 (SOD1) allele; however, the genetic examination for the SOD1 mutation does not exclusively detect symptomatic dogs. In order to identify novel biomarkers, the plasma microRNA (miRNA) profiles of PWCs with DM were investigated.

Results: Quantification of the plasma levels of 277 miRNAs by an RT-qPCR array identified 11 up-regulated miRNAs and 7 down-regulated miRNAs in DM-affected PWCs from those in wild-type SOD1 PWCs. A pathway analysis identified 3 miRNAs: miR-26b, miR-181a, and miR-196a, which potentially regulate several genes associated with SOD1. In order to validate the diagnostic accuracy of the candidate miRNAs in the aged PWC population, candidate miRNAs in plasma were measured by RT-qPCR and a receiver operating characteristic (ROC) curve analysis was performed. miR-26b had the largest area under the ROC curve for distinguishing DM PWCs from healthy PWCs (sensitivity, 66.7%; specificity, 87.0%). The plasma level of miR-26b was significantly higher in the DM group than in the healthy control group. A positive correlation was observed between increases in the plasma level of miR-26b and disease progression.

Conclusions: These results suggest that plasma miR-26b is a potential novel diagnostic biomarker of DM.

Citing Articles

Genotypic and Allelic Frequencies of Degenerative Myelopathy in an Italian Canine Population.

Ghilardi S, Minozzi G, De Iorio M, Gonzi C, Frattini S, Bagardi M Animals (Basel). 2024; 14(18).

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A review on microRNA detection and expression studies in dogs.

Varvil M, Dos Santos A Front Vet Sci. 2023; 10:1261085.

PMID: 37869503 PMC: 10585042. DOI: 10.3389/fvets.2023.1261085.

Evaluation of serum miR-216a and miR-375 as biomarkers in dogs with acute pancreatitis.

Lee D, Yun T, Koo Y, Chae Y, Choi M, Kang B J Vet Intern Med. 2022; 37(1):92-100.

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Electrical Impedance Myography in Dogs With Degenerative Myelopathy.

Kowal J, Verga S, Pandeya S, Cochran R, Sabol J, Rutkove S Front Vet Sci. 2022; 9:874277.

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Brain diffusion tensor imaging in dogs with degenerative myelopathy.

Lewis M, Shomper J, Williamson B, Vansteenkiste D, Bibi K, Lim S J Vet Intern Med. 2021; 35(5):2342-2349.

PMID: 34410026 PMC: 8478048. DOI: 10.1111/jvim.16248.

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