Murine Osteoblastlike Cells and the Osteogenic Cell MC3T3-E1 Release a Macrophage Colony-stimulating Activity in Culture

Overview

Journal Calcif Tissue Int

Specialty Pathology

Date 1987 Sep 1

PMID 3117342

Citations 22

Authors

P R Elford

R Felix

M Cecchini

U Trechsel

H Fleisch

Affiliations

Soon will be listed here.

Abstract

The osteoclast may be of hematopoietic lineage and as such its development could be regulated by colony-stimulating factors. Since there is much interest as to whether osteoblasts influence bone resorption, we examined whether bone cells produce colony-stimulating activity. Both cells isolated from neonatal calvaria and the osteogenic cell MC3T3-E1 were found to constitutively release a colony-stimulating activity possessing characteristics of a macrophage colony-stimulating factor, as determined by basic biochemical purification and by identity of colonies induced in cultures of bone marrow cells. Release could be increased by the presence of the bone-resorbing agents lipopolysaccharide and 1,25 dihydroxyvitamin D3. We conclude that the osteoblast may contribute to both the processes of osteoclast formation and of hematopoiesis through the secretion of colony-stimulating activity into the adjacent bone marrow.

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