Human Osteoblast-like Cells Expressing MHC Class II Determinants Stimulate Allogeneic and Autologous Peripheral Blood Mononuclear Cells and Function As Antigen-presenting Cells

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1989 Nov 1

PMID 2480330

Citations 7

Authors

H Skjodt

T Moller

S F Freiesleben

Affiliations

Soon will be listed here.

Abstract

We have previously shown that a subset of human osteoblast-like bone surface cells (HBC) derived from explants of trabecular bone constitutively express class II major histocompatibility complex (MHC) determinants in long-term culture. We now examine the capacity of HBC to stimulate allogeneic and autologous peripheral blood mononuclear cells (PBMC) and to function as antigen-presenting cells (APC). HBC consistently stimulated a strong proliferative response in allogeneic PBMC after 6 days co-culture. Stimulation of autologous PBMC was weaker and less consistent. HBC and adherent PBMC were equally active in stimulating a PPD response in autologous non-adherent PBMC. These observations suggest that immune cells initiated by HBC may play a role in normal and disordered bone remodelling processes.

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