ANO1/TMEM16A Regulates Process Maturation in Radial Glial Cells in the Developing Brain

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2019 Jun 1

PMID 31147466

Citations 14

Authors

Gyu-Sang Hong

Sung Hoon Lee

Byeongjun Lee

Jae Hyouk Choi

Soo-Jin Oh

Yongwoo Jang

Eun Mi Hwang

Hyungsup Kim

Jooyoung Jung

In-Beom Kim

Uhtaek Oh

Affiliations

Soon will be listed here.

Abstract

Neural stem cells (NSCs) are primary progenitor cells in the early developmental stage in the brain that initiate a diverse lineage of differentiated neurons and glia. Radial glial cells (RGCs), a type of neural stem cell in the ventricular zone, are essential for nurturing and delivering new immature neurons to the appropriate cortical target layers. Here we report that Anoctamin 1 (ANO1)/TMEM16A, a Ca-activated chloride channel, mediates the Ca-dependent process extension of RGCs. ANO1 is highly expressed and functionally active in RGCs of the mouse embryonic ventricular zone. Knockdown of ANO1 suppresses RGC process extension and protrusions, whereas ANO1 overexpression stimulates process extension. Among various trophic factors, brain-derived neurotrophic factor (BDNF) activates ANO1, which is required for BDNF-induced process extension in RGCs. More importantly, -deficient mice exhibited disrupted cortical layers and reduced cortical thickness. We thus conclude that the regulation of RGC process extension by ANO1 contributes to the normal formation of mouse embryonic brain.

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