Isolation and Characterization of Clinical in Beijing, China, 2014-2016

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2019 May 30

PMID 31139159

Citations 19

Authors

Xiaoai Zhang

Yanlin Niu

Yuzhu Liu

Zheng Lu

Di Wang

Xia Cui

Qian Chen

Xiaochen Ma

Affiliations

Soon will be listed here.

Abstract

is an important foodborne pathogen with a significant impact on public health worldwide. A great number of outbreaks caused by has been reported, especially in the United States, and European countries. However, listeriosis has not yet been included in notifiable disease in China, and thus information on this infection has been scarce among the Chinese population. In this study, we described a 3-year surveillance of listeriosis in Beijing, China. Fifty-six strains isolated from 49 clinical infectious cases (27 pregnancy-associated infections and 22 non-pregnancy-associated infections) were analyzed by serotyping, pulsed field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and antimicrobial susceptibility testing between 2014 and 2016 in Beijing. The predominant serogroups were 1/2a,3a and 1/2b,3b,7 which accounted for 92% of the overall isolates. Four strains were serogroup 4b,4d,4e, isolated from patients with pregnancy-associated infections. Based on PFGE, these isolates were divided into 32 pulsotypes (PTs) and 3 clusters associated with serogroups. Ten PTs were represented by more than one isolate with PT09 containing the most number of isolates. MLST differentiated the isolates into 18 STs, without new ST designated. The three most common STs were ST8 (18.4%), ST5 (16.3%), and ST87 (12.2%), accounting for 46.9% of the isolates. STs prevalent in other parts of the world were also present in China such as ST1, ST2, ST5, ST8, and ST9 which caused maternal fetal infections or outbreaks. However, the STs and serogroup distribution of clinical in Beijing, China was different from those in other countries. Strains of ST1 and ST2 were isolated from patients with pregnancy-associated infection, whereas none of ST155 isolates caused pregnancy-associated cases. Surveillance of molecular characterization will provide important information for prevention of listeriosis. This study also enhances our understanding of genetic diversity of clinical in China.

Citing Articles

Comparative Analysis of and Virulence Among Foodborne and Clinical Strains.

Yan H, Xu B, Gao B, Xu Y, Xia X, Ma Y Microorganisms. 2025; 13(1).

PMID: 39858959 PMC: 11767709. DOI: 10.3390/microorganisms13010191.

Genetic diversity of from seafood products, its processing environment, and clinical origin in the Western Cape, South Africa using whole genome sequencing.

Lambrechts K, Gouws P, Rip D AIMS Microbiol. 2024; 10(3):608-643.

PMID: 39219753 PMC: 11362271. DOI: 10.3934/microbiol.2024029.

Enterocins Produced by Enterococci Isolated from Breast-Fed Infants: Antilisterial Potential.

Landete J, Montiel R, Rodriguez-Minguez E, Arques J Children (Basel). 2024; 11(2).

PMID: 38397373 PMC: 10887673. DOI: 10.3390/children11020261.

Analysis of Clinical and Microbiological Features of Infection.

Lu X, Yang H, Wang Y, Xie Y Infect Drug Resist. 2023; 16:2793-2803.

PMID: 37187483 PMC: 10179002. DOI: 10.2147/IDR.S408089.

Genomic Characterization of a Clinical ST1 Isolate Recovered from the Blood Sample of a Woman with Third Trimester Stillbirth.

Ge Y, Xu G, Ruan Z, Wang Y Infect Drug Resist. 2022; 15:5529-5532.

PMID: 36158236 PMC: 9507440. DOI: 10.2147/IDR.S384589.

References

Zhang J, Cao G, Xu X, Allard M, Li P, Brown E . Evolution and Diversity of Listeria monocytogenes from Clinical and Food Samples in Shanghai, China. Front Microbiol. 2016; 7:1138. PMC: 4956650. DOI: 10.3389/fmicb.2016.01138. View

Graves L, Swaminathan B . PulseNet standardized protocol for subtyping Listeria monocytogenes by macrorestriction and pulsed-field gel electrophoresis. Int J Food Microbiol. 2001; 65(1-2):55-62. DOI: 10.1016/s0168-1605(00)00501-8. View

Ragon M, Wirth T, Hollandt F, Lavenir R, Lecuit M, Monnier A . A new perspective on Listeria monocytogenes evolution. PLoS Pathog. 2008; 4(9):e1000146. PMC: 2518857. DOI: 10.1371/journal.ppat.1000146. View

Linnan M, Mascola L, Lou X, Goulet V, May S, Salminen C . Epidemic listeriosis associated with Mexican-style cheese. N Engl J Med. 1988; 319(13):823-8. DOI: 10.1056/NEJM198809293191303. View

Jackson B, Salter M, Tarr C, Conrad A, Harvey E, Steinbock L . Notes from the field: listeriosis associated with stone fruit--United States, 2014. MMWR Morb Mortal Wkly Rep. 2015; 64(10):282-3. PMC: 4584806. View

Doumith M, Buchrieser C, Glaser P, Jacquet C, Martin P . Differentiation of the major Listeria monocytogenes serovars by multiplex PCR. J Clin Microbiol. 2004; 42(8):3819-22. PMC: 497638. DOI: 10.1128/JCM.42.8.3819-3822.2004. View

Chen M, Cheng J, Wu Q, Zhang J, Chen Y, Xue L . Occurrence, Antibiotic Resistance, and Population Diversity of Isolated From Fresh Aquatic Products in China. Front Microbiol. 2018; 9:2215. PMC: 6157410. DOI: 10.3389/fmicb.2018.02215. View

Magalhaes R, Ferreira V, Santos I, Almeida G, Teixeira P . Genetic and phenotypic characterization of Listeria monocytogenes from human clinical cases that occurred in Portugal between 2008 and 2012. Foodborne Pathog Dis. 2014; 11(11):907-16. DOI: 10.1089/fpd.2014.1806. View

Althaus D, Jermini M, Giannini P, Martinetti G, Reinholz D, Nuesch-Inderbinen M . Local Outbreak of Listeria monocytogenes Serotype 4b Sequence Type 6 Due to Contaminated Meat Pâté. Foodborne Pathog Dis. 2017; 14(4):219-222. DOI: 10.1089/fpd.2016.2232. View

10.

Gottlieb S, Newbern E, Griffin P, Graves L, Hoekstra R, Baker N . Multistate outbreak of Listeriosis linked to turkey deli meat and subsequent changes in US regulatory policy. Clin Infect Dis. 2005; 42(1):29-36. DOI: 10.1086/498113. View

11.

Lv J, Qin Z, Xu Y, Xie Q . Listeria infection in Chinese pregnant women and neonates from Shandong. Int J Clin Exp Med. 2014; 7(9):2730-4. PMC: 4211782. View

12.

Goulet V, Hedberg C, Monnier A, De Valk H . Increasing incidence of listeriosis in France and other European countries. Emerg Infect Dis. 2008; 14(5):734-40. PMC: 2600261. DOI: 10.3201/eid1405.071395. View

13.

Schlech 3rd W, Lavigne P, Bortolussi R, Allen A, Haldane E, Wort A . Epidemic listeriosis--evidence for transmission by food. N Engl J Med. 1983; 308(4):203-6. DOI: 10.1056/NEJM198301273080407. View

14.

McCollum J, Cronquist A, Silk B, Jackson K, OConnor K, Cosgrove S . Multistate outbreak of listeriosis associated with cantaloupe. N Engl J Med. 2013; 369(10):944-53. DOI: 10.1056/NEJMoa1215837. View

15.

Verghese B, Lok M, Wen J, Alessandria V, Chen Y, Kathariou S . comK prophage junction fragments as markers for Listeria monocytogenes genotypes unique to individual meat and poultry processing plants and a model for rapid niche-specific adaptation, biofilm formation, and persistence. Appl Environ Microbiol. 2011; 77(10):3279-92. PMC: 3126449. DOI: 10.1128/AEM.00546-11. View

16.

Jadhav S, Bhave M, Palombo E . Methods used for the detection and subtyping of Listeria monocytogenes. J Microbiol Methods. 2012; 88(3):327-41. DOI: 10.1016/j.mimet.2012.01.002. View

17.

Dalton C, Austin C, Sobel J, Hayes P, Bibb W, Graves L . An outbreak of gastroenteritis and fever due to Listeria monocytogenes in milk. N Engl J Med. 1997; 336(2):100-5. DOI: 10.1056/NEJM199701093360204. View

18.

Salcedo C, Arreaza L, Alcala B, de la Fuente L, Vazquez J . Development of a multilocus sequence typing method for analysis of Listeria monocytogenes clones. J Clin Microbiol. 2003; 41(2):757-62. PMC: 149676. DOI: 10.1128/JCM.41.2.757-762.2003. View

19.

Wang Y, Jiao Y, Lan R, Xu X, Liu G, Wang X . Characterization of Listeria monocytogenes isolated from human Listeriosis cases in China. Emerg Microbes Infect. 2015; 4(8):e50. PMC: 4576168. DOI: 10.1038/emi.2015.50. View

20.

Lomonaco S, Verghese B, Gerner-Smidt P, Tarr C, Gladney L, Joseph L . Novel epidemic clones of Listeria monocytogenes, United States, 2011. Emerg Infect Dis. 2012; 19(1):147-50. PMC: 3558006. DOI: 10.3201/eid1901.121167. View