Synergistically Enhanced Colorimetric Molecular Detection Using Smart Cup: a Case for Instrument-free HPV-associated Cancer Screening

Overview

Journal Theranostics

Date 2019 May 28

PMID 31131058

Citations 22

Authors

Kun Yin

Vikram Pandian

Karteek Kadimisetty

Carlos Ruiz

Kumarasen Cooper

Jianxin You

Changchun Liu

Affiliations

Soon will be listed here.

Abstract

: Early and accurate detection of disease is crucial for its prevention, identification, and treatment. However, most of disease diagnostics is still limited in clinical laboratories due to the need of complicated instruments and professional personnel. Herein, we reported a smartphone-based synergistically enhanced colorimetric method for molecular diagnostics in our point of care (POC) smart cup platform. : A disposable microfluidic chip was developed for colorimetric loop-mediated isothermal amplification (LAMP) detection of multiple HPV DNA in our POC smart cup platform. The colorimetric detection takes advantage of synergistic effect of PPi and H ions, two byproducts of LAMP reaction. Color signal of LAMP assay was recorded and analyzed by our custom Android app (dubbed "Hue Analyzer"). : Our method not only significantly improves colorimetric readout, but also provides a 10-fold increase in detection sensitivity. It has been successfully applied for HPV-associated cancer screening with spiked saliva and clinical swab samples. : The proposed POC diagnostic platform is completely compatible with other nucleic acid biomarkers and has great potential for personalized health monitoring and disease prevention.

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