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Evaluation of Colorimetric Detection Methods for Shigella, Salmonella, and Vibrio Cholerae by Loop-mediated Isothermal Amplification

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Date 2013 Oct 26
PMID 24157057
Citations 15
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Abstract

We evaluated loop-mediated isothermal amplification end-point detection methods for Salmonella, Shigella, and Vibrio cholerae. Detection sensitivities were comparable to real-time PCR methods. The colorimetric dyes hydroxynaphthol blue and SYBR Green I showed increased sensitivity when compared to visual and automated turbidity readings. End-point colorimetric dyes promise great utility in developing settings.

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