» Articles » PMID: 31123908

Novel MiRNAs As Potential Biomarkers in Stage II Colon Cancer: Microarray Analysis

Overview
Journal Mol Biol Rep
Specialty Molecular Biology
Date 2019 May 25
PMID 31123908
Citations 27
Authors
Affiliations
Soon will be listed here.
Abstract

The aim of this study was to determine oncogenic and tumor-suppressing miRNA profiles associated with the development and progression of cancer using tumor tissues from patients with colorectal cancer (stage II) that did not show nodal spread or advanced metastasis to identify potential biomarkers. A microarray system (GeneChip miRNA 4.0 Array chip, Affymetrix) was used to determine the microRNA profiles of five patients with stage II colon cancer based on normal and colon tumor tissues. Of 32 identified miRNAs, an increase in three microRNAs (hsa-miR-4745-5p, hsa-miR-6126, and hsa-miR-1469) was observed in tumor tissues relative to that in control tissues. Additionally, this study demonstrated for the first time that the expression of the 8 miRNAs (hsa-miR-378i, hsa-miR-378a-3p, hsa-miR-378c, hsa-miR-378d, hsa-miR-378e, hsa-miR-378f, hsa-miR-378a-5p, and hsa-miR-378g) from miR-378 members among the differentially expressed miRNAs is reduced. The target genes of these downregulated miRNAs were determined by using DIANA miRPath v3. The effect of identified genes on colon cancer stage II was determined the biological process and biological pathway using Funrich Gene Enrichment. It was revealed that these miRNAs were affected the signaling pathways which control cell proliferation, cell-cell interaction, and apoptosis in stage II colon cancer. In patients with early stage II colon cancer, miR-378 can be used as a biomarker of colorectal cancer. Thus, miR-378 can facilitate treatment with early diagnosis.

Citing Articles

FOXD2-AS1 promotes malignant cell behavior in oral squamous cell carcinoma via the miR-378 g/CRABP2 axis.

Guo S, Huang B, You Z, Luo Z, Xu D, Zhang J BMC Oral Health. 2024; 24(1):625.

PMID: 38807101 PMC: 11134640. DOI: 10.1186/s12903-024-04388-2.


Identification, characterization and expression analysis of circRNA encoded by SARS-CoV-1 and SARS-CoV-2.

Niu M, Wang C, Chen Y, Zou Q, Xu L Brief Bioinform. 2024; 25(2).

PMID: 38279648 PMC: 10818166. DOI: 10.1093/bib/bbad537.


LUM as a novel prognostic marker and its correlation with immune infiltration in gastric cancer: a study based on immunohistochemical analysis and bioinformatics.

Xu W, Chen S, Jiang Q, He J, Zhang F, Wang Z BMC Gastroenterol. 2023; 23(1):455.

PMID: 38129820 PMC: 10740220. DOI: 10.1186/s12876-023-03075-w.


Identifying potential circulating miRNA biomarkers for the diagnosis and prediction of ovarian cancer using machine-learning approach: application of Boruta.

Hamidi F, Gilani N, Arabi Belaghi R, Yaghoobi H, Babaei E, Sarbakhsh P Front Digit Health. 2023; 5:1187578.

PMID: 37621964 PMC: 10445490. DOI: 10.3389/fdgth.2023.1187578.


Integrative Transcriptomic Profiling of the Wilms Tumor.

Avcin S, crepinsek K, Jenko Bizjan B, Sket R, Kovac J, Vrhovsek B Cancers (Basel). 2023; 15(15).

PMID: 37568662 PMC: 10416970. DOI: 10.3390/cancers15153846.


References
1.
Bartel D . MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004; 116(2):281-97. DOI: 10.1016/s0092-8674(04)00045-5. View

2.
Iorio M, Ferracin M, Liu C, Veronese A, Spizzo R, Sabbioni S . MicroRNA gene expression deregulation in human breast cancer. Cancer Res. 2005; 65(16):7065-70. DOI: 10.1158/0008-5472.CAN-05-1783. View

3.
Pillai R . MicroRNA function: multiple mechanisms for a tiny RNA?. RNA. 2005; 11(12):1753-61. PMC: 1370863. DOI: 10.1261/rna.2248605. View

4.
Akao Y, Nakagawa Y, Naoe T . let-7 microRNA functions as a potential growth suppressor in human colon cancer cells. Biol Pharm Bull. 2006; 29(5):903-6. DOI: 10.1248/bpb.29.903. View

5.
Saito Y, Liang G, Egger G, Friedman J, Chuang J, Coetzee G . Specific activation of microRNA-127 with downregulation of the proto-oncogene BCL6 by chromatin-modifying drugs in human cancer cells. Cancer Cell. 2006; 9(6):435-43. DOI: 10.1016/j.ccr.2006.04.020. View