Standardization of Methods to Quantify and Culture Endothelial Colony-forming Cells Derived from Peripheral Blood: Position Paper from the International Society on Thrombosis and Haemostasis SSC

Overview

Journal J Thromb Haemost

Publisher Elsevier

Specialty Hematology

Date 2019 May 24

PMID 31119878

Citations 33

Authors

David M Smadja

Juan M Melero-Martin

Jeroen Eikenboom

Mackenzie Bowman

Florence Sabatier

Anna M Randi

Affiliations

Soon will be listed here.

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References

Starke R, Ferraro F, Paschalaki K, Dryden N, McKinnon T, Sutton R . Endothelial von Willebrand factor regulates angiogenesis. Blood. 2010; 117(3):1071-80. PMC: 3035068. DOI: 10.1182/blood-2010-01-264507. View

Naito H, Kidoya H, Sakimoto S, Wakabayashi T, Takakura N . Identification and characterization of a resident vascular stem/progenitor cell population in preexisting blood vessels. EMBO J. 2011; 31(4):842-55. PMC: 3280559. DOI: 10.1038/emboj.2011.465. View

Melero-Martin J, De Obaldia M, Kang S, Khan Z, Yuan L, Oettgen P . Engineering robust and functional vascular networks in vivo with human adult and cord blood-derived progenitor cells. Circ Res. 2008; 103(2):194-202. PMC: 2746761. DOI: 10.1161/CIRCRESAHA.108.178590. View

Bacha N, Blandinieres A, Rossi E, Gendron N, Nevo N, Lecourt S . Endothelial Microparticles are Associated to Pathogenesis of Idiopathic Pulmonary Fibrosis. Stem Cell Rev Rep. 2017; 14(2):223-235. DOI: 10.1007/s12015-017-9778-5. View

Ingram D, Mead L, Tanaka H, Meade V, Fenoglio A, Mortell K . Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood. Blood. 2004; 104(9):2752-60. DOI: 10.1182/blood-2004-04-1396. View

Levy M, Bonnet D, Mauge L, Celermajer D, Gaussem P, Smadja D . Circulating endothelial cells in refractory pulmonary hypertension in children: markers of treatment efficacy and clinical worsening. PLoS One. 2013; 8(6):e65114. PMC: 3677895. DOI: 10.1371/journal.pone.0065114. View

Reid E, Guduric-Fuchs J, ONeill C, Allen L, Chambers S, Stitt A . Preclinical Evaluation and Optimization of a Cell Therapy Using Human Cord Blood-Derived Endothelial Colony-Forming Cells for Ischemic Retinopathies. Stem Cells Transl Med. 2017; 7(1):59-67. PMC: 5746158. DOI: 10.1002/sctm.17-0187. View

Yoder M, Mead L, Prater D, Krier T, Mroueh K, Li F . Redefining endothelial progenitor cells via clonal analysis and hematopoietic stem/progenitor cell principals. Blood. 2006; 109(5):1801-9. PMC: 1801067. DOI: 10.1182/blood-2006-08-043471. View

Jarajapu Y, Hazra S, Segal M, Li Calzi S, LiCalzi S, Jadhao C . Vasoreparative dysfunction of CD34+ cells in diabetic individuals involves hypoxic desensitization and impaired autocrine/paracrine mechanisms. PLoS One. 2014; 9(4):e93965. PMC: 3979711. DOI: 10.1371/journal.pone.0093965. View

10.

Lee S, Lee J, Asahara T, Kim Y, Jeong H, Ahn Y . Genistein promotes endothelial colony-forming cell (ECFC) bioactivities and cardiac regeneration in myocardial infarction. PLoS One. 2014; 9(5):e96155. PMC: 4022670. DOI: 10.1371/journal.pone.0096155. View

11.

Smadja D, Mauge L, Nunes H, dAudigier C, Juvin K, Borie R . Imbalance of circulating endothelial cells and progenitors in idiopathic pulmonary fibrosis. Angiogenesis. 2012; 16(1):147-57. DOI: 10.1007/s10456-012-9306-9. View

12.

Mauge L, Sabatier F, Boutouyrie P, dAudigier C, Peyrard S, Bozec E . Forearm ischemia decreases endothelial colony-forming cell angiogenic potential. Cytotherapy. 2013; 16(2):213-24. DOI: 10.1016/j.jcyt.2013.09.007. View

13.

Paschalaki K, Randi A . Recent Advances in Endothelial Colony Forming Cells Toward Their Use in Clinical Translation. Front Med (Lausanne). 2018; 5:295. PMC: 6205967. DOI: 10.3389/fmed.2018.00295. View

14.

Fang S, Wei J, Pentinmikko N, Leinonen H, Salven P . Generation of functional blood vessels from a single c-kit+ adult vascular endothelial stem cell. PLoS Biol. 2012; 10(10):e1001407. PMC: 3473016. DOI: 10.1371/journal.pbio.1001407. View

15.

Paschalaki K, Starke R, Hu Y, Mercado N, Margariti A, Gorgoulis V . Dysfunction of endothelial progenitor cells from smokers and chronic obstructive pulmonary disease patients due to increased DNA damage and senescence. Stem Cells. 2013; 31(12):2813-26. PMC: 4377082. DOI: 10.1002/stem.1488. View

16.

Randi A, Laffan M . Von Willebrand factor and angiogenesis: basic and applied issues. J Thromb Haemost. 2016; 15(1):13-20. DOI: 10.1111/jth.13551. View

17.

Simoncini S, Chateau A, Robert S, Todorova D, Yzydorzick C, Lacroix R . Biogenesis of Pro-senescent Microparticles by Endothelial Colony Forming Cells from Premature Neonates is driven by SIRT1-Dependent Epigenetic Regulation of MKK6. Sci Rep. 2017; 7(1):8277. PMC: 5557933. DOI: 10.1038/s41598-017-08883-1. View

18.

Munoz-Hernandez R, Miranda M, Stiefel P, Lin R, Praena-Fernandez J, Dominguez-Simeon M . Decreased level of cord blood circulating endothelial colony-forming cells in preeclampsia. Hypertension. 2014; 64(1):165-71. PMC: 4057301. DOI: 10.1161/HYPERTENSIONAHA.113.03058. View

19.

Lin R, Moreno-Luna R, Li D, Jaminet S, Greene A, Melero-Martin J . Human endothelial colony-forming cells serve as trophic mediators for mesenchymal stem cell engraftment via paracrine signaling. Proc Natl Acad Sci U S A. 2014; 111(28):10137-42. PMC: 4104912. DOI: 10.1073/pnas.1405388111. View

20.

Starke R, Paschalaki K, Dyer C, Harrison-Lavoie K, Cutler J, McKinnon T . Cellular and molecular basis of von Willebrand disease: studies on blood outgrowth endothelial cells. Blood. 2013; 121(14):2773-84. PMC: 3617637. DOI: 10.1182/blood-2012-06-435727. View