Phytofabrication of Selenium Nanoparticles From Fruit Extract and Exploring Its Biopotential Applications: Antioxidant, Antimicrobial, and Biocompatibility

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2019 May 23

PMID 31114564

Citations 94

Authors

Lokanadhan Gunti

Regina Sharmila Dass

Naveen Kumar Kalagatur

Affiliations

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Abstract

In the present study, phytofabricated selenium nanoparticles (PF-SeNPs) were prepared from aqueous fruit extract of in a facile, green, economic, tactic and eco-friendly way. The aqueous fruit extract of was found to be rich with various secondary metabolites including phenolics (59.18 ± 2.91 mg gallic acid equivalents/g), flavonoids (38.50 ± 2.84 mg catechin equivalents/g), and tannins (44.28 ± 3.09 mg tannic acid equivalents/g) and determined that highly appropriate for the biosynthesis of nanoparticles. The facile phytofabrication of PF-SeNPs was confirmed by UV-visible and FTIR spectroscopic analysis. The XRD pattern and Raman spectroscopy showed that synthesized PF-SeNPs were amorphous in nature. The Zeta potential analysis confirmed that PF-SeNPs were negatively charged (-24.4 mV). The DLS analysis revealed that PF-SeNPs were in nano size and less aggregated with poly-dispersity index of less than 0.2. The SEM images depicted that PF-SeNPs were spherical in shape. The EDX analysis revealed that PF-SeNPs were constituted with Se (61.60%), C (29.96%), and O (4.41%). The HR-TEM analysis determined that PF-SeNPs were in nano size with an average diameter of 15-40 nm. The PF-SeNPs have offered fascinating bio-potential applications, such as antioxidant, antimicrobial and biocompatibility. They have also exhibited dose-dependent free radical scavenging activity, and EC50 was determined as 15.67 ± 1.41 and 18.84 ± 1.02 μg/mL for DPPH and ABTS assays, respectively. The PF-SeNPs has also shown the wide range of antimicrobial activity on foodborne pathogens, and it was found to be highly efficient on fungi followed by Gram-positive and Gram-negative bacteria. The biocompatibility of PF-SeNPs was assessed in N2a cells with much higher IC50 value (dose required to inhibit 50% of cell viability) compared to sodium selenite. Also, mitochondrial membrane potential (MMP) and caspase-3 were much less altered on treatment of PF-SeNPs related to sodium selenite. The cytotoxic studies clearly determined that PF-SeNPs was much less toxic and safer related to sodium selenite. Thus, PF-SeNPs could find suitable application as antioxidant and antimicrobial agent in food, biomedical, and pharmaceutical industry.

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References

Porter A, Janicke R . Emerging roles of caspase-3 in apoptosis. Cell Death Differ. 1999; 6(2):99-104. DOI: 10.1038/sj.cdd.4400476. View

Kimura K, Hane Y, Watanabe Y . Effect of pre-coagulation on mitigating irreversible fouling during ultrafiltration of a surface water. Water Sci Technol. 2005; 51(6-7):93-100. View

Ankamwar B, Damle C, Ahmad A, Sastry M . Biosynthesis of gold and silver nanoparticles using Emblica Officinalis fruit extract, their phase transfer and transmetallation in an organic solution. J Nanosci Nanotechnol. 2005; 5(10):1665-71. DOI: 10.1166/jnn.2005.184. View

Rayman M . Selenium in cancer prevention: a review of the evidence and mechanism of action. Proc Nutr Soc. 2005; 64(4):527-42. DOI: 10.1079/pns2005467. View

Bryden W . Mycotoxins in the food chain: human health implications. Asia Pac J Clin Nutr. 2007; 16 Suppl 1:95-101. View

Pierard G, Arrese J, Pierard-Franchimont C, De Doncker P . Prolonged effects of antidandruff shampoos - time to recurrence of Malassezia ovalis colonization of skin. Int J Cosmet Sci. 1997; 19(3):111-7. DOI: 10.1046/j.1467-2494.1997.171706.x. View

Chen T, Wong Y, Zheng W, Bai Y, Huang L . Selenium nanoparticles fabricated in Undaria pinnatifida polysaccharide solutions induce mitochondria-mediated apoptosis in A375 human melanoma cells. Colloids Surf B Biointerfaces. 2008; 67(1):26-31. DOI: 10.1016/j.colsurfb.2008.07.010. View

Li W, Xie X, Shi Q, Zeng H, Ou-Yang Y, Chen Y . Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli. Appl Microbiol Biotechnol. 2009; 85(4):1115-22. DOI: 10.1007/s00253-009-2159-5. View

Zheng J, Zheng S, Zhang Y, Yu B, Zheng W, Yang F . Sialic acid surface decoration enhances cellular uptake and apoptosis-inducing activity of selenium nanoparticles. Colloids Surf B Biointerfaces. 2010; 83(1):183-7. DOI: 10.1016/j.colsurfb.2010.11.023. View

10.

Battin E, Zimmerman M, Ramoutar R, Quarles C, Brumaghim J . Preventing metal-mediated oxidative DNA damage with selenium compounds. Metallomics. 2011; 3(5):503-12. DOI: 10.1039/c0mt00063a. View

11.

Park Y, Hong Y, Weyers A, Kim Y, Linhardt R . Polysaccharides and phytochemicals: a natural reservoir for the green synthesis of gold and silver nanoparticles. IET Nanobiotechnol. 2011; 5(3):69-78. DOI: 10.1049/iet-nbt.2010.0033. View

12.

El-Wahab H, Moram G . Toxic effects of some synthetic food colorants and/or flavor additives on male rats. Toxicol Ind Health. 2012; 29(2):224-32. DOI: 10.1177/0748233711433935. View

13.

Yang F, Tang Q, Zhong X, Bai Y, Chen T, Zhang Y . Surface decoration by Spirulina polysaccharide enhances the cellular uptake and anticancer efficacy of selenium nanoparticles. Int J Nanomedicine. 2012; 7:835-44. PMC: 3284226. DOI: 10.2147/IJN.S28278. View

14.

Yu B, Zhang Y, Zheng W, Fan C, Chen T . Positive surface charge enhances selective cellular uptake and anticancer efficacy of selenium nanoparticles. Inorg Chem. 2012; 51(16):8956-63. DOI: 10.1021/ic301050v. View

15.

Golechha M, Bhatia J, Arya D . Studies on effects of Emblica officinalis (Amla) on oxidative stress and cholinergic function in scopolamine induced amnesia in mice. J Environ Biol. 2012; 33(1):95-100. View

16.

Prasad K, Patel H, Patel T, Patel K, Selvaraj K . Biosynthesis of Se nanoparticles and its effect on UV-induced DNA damage. Colloids Surf B Biointerfaces. 2012; 103:261-6. DOI: 10.1016/j.colsurfb.2012.10.029. View

17.

Ramamurthy C, Sampath K, Arunkumar P, Suresh Kumar M, Sujatha V, Premkumar K . Green synthesis and characterization of selenium nanoparticles and its augmented cytotoxicity with doxorubicin on cancer cells. Bioprocess Biosyst Eng. 2013; 36(8):1131-9. DOI: 10.1007/s00449-012-0867-1. View

18.

Kazempour Z, Yazdi M, Rafii F, Shahverdi A . Sub-inhibitory concentration of biogenic selenium nanoparticles lacks post antifungal effect for Aspergillus niger and Candida albicans and stimulates the growth of Aspergillus niger. Iran J Microbiol. 2013; 5(1):81-5. PMC: 3577562. View

19.

Iranifam M, Fathinia M, Sadeghi Rad T, Hanifehpour Y, Khataee A, Joo S . A novel selenium nanoparticles-enhanced chemiluminescence system for determination of dinitrobutylphenol. Talanta. 2013; 107:263-9. DOI: 10.1016/j.talanta.2012.12.043. View

20.

Huang Y, He L, Liu W, Fan C, Zheng W, Wong Y . Selective cellular uptake and induction of apoptosis of cancer-targeted selenium nanoparticles. Biomaterials. 2013; 34(29):7106-16. DOI: 10.1016/j.biomaterials.2013.04.067. View