The Dodecahedral Framework of the Bacteriophage Phi 6 Nucleocapsid is Composed of Protein P1

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1987 Aug 1

PMID 3110430

Citations 15

Authors

N T Ktistakis

D Lang

Affiliations

Soon will be listed here.

Abstract

The outer layer of the bacteriophage phi 6 nucleocapsid (NC) was removed by EDTA and reassociated with the core in the presence of Ca2+ or Mg2+. The core was relatively inaccessible to trypsin digestion, was composed of protein P1, and was in the dodecahedral framework reported previously. (H.T. Steely, Jr., and D. Lang, J. Virol. 51:479-483, 1984; Y. Yang and D. Lang, J. Virol. 51:484-488, 1984). The double-stranded RNA genome became RNase sensitive after EDTA treatment of the nucleocapsid.

Citing Articles

Diversity and Current Classification of dsRNA Bacteriophages.

Mantynen S, Salomaa M, Poranen M Viruses. 2023; 15(11).

PMID: 38005832 PMC: 10674327. DOI: 10.3390/v15112154.

RNA Packaging in the Bacteriophages: Dynamic Interactions during Capsid Maturation.

Gottlieb P, Alimova A Int J Mol Sci. 2022; 23(5).

PMID: 35269819 PMC: 8910881. DOI: 10.3390/ijms23052677.

Nonspecific nucleoside triphosphatase P4 of double-stranded RNA bacteriophage phi6 is required for single-stranded RNA packaging and transcription.

Pirttimaa M, Paatero A, Frilander M, Bamford D J Virol. 2002; 76(20):10122-7.

PMID: 12239286 PMC: 136547. DOI: 10.1128/jvi.76.20.10122-10127.2002.

A novel virus-host cell membrane interaction. Membrane voltage-dependent endocytic-like entry of bacteriophage straight phi6 nucleocapsid.

Poranen M, Daugelavicius R, Ojala P, Hess M, Bamford D J Cell Biol. 1999; 147(3):671-82.

PMID: 10545509 PMC: 2151191. DOI: 10.1083/jcb.147.3.671.

Packaging and replication regulation revealed by chimeric genome segments of double-stranded RNA bacteriophage phi6.

Poranen M, Bamford D RNA. 1999; 5(3):446-54.

PMID: 10094312 PMC: 1369772. DOI: 10.1017/s1355838299981876.

References

Henderson C, Perham R, Finch J . Structure and symmetry of B. stearothermophilus pyruvate dehydrogenase multienzyme complex and implications for eucaryote evolution. Cell. 1979; 17(1):85-93. DOI: 10.1016/0092-8674(79)90297-6. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Mindich L, Sinclair J, Cohen J . The morphogenesis of bacteriophage phi6: particles formed by nonsense mutants. Virology. 1976; 75(1):224-31. DOI: 10.1016/0042-6822(76)90021-0. View

Steely Jr H, Lang D . Electron microscopy of bacteriophage phi 6 nucleocapsid: two-dimensional image analysis. J Virol. 1984; 51(2):479-83. PMC: 254462. DOI: 10.1128/JVI.51.2.479-483.1984. View

Etten J, Lane L, Gonzalez C, Partridge J, Vidaver A . Comparative properties of bacteriophage phi6 and phi6 nucleocapsid. J Virol. 1976; 18(2):652-8. PMC: 515592. DOI: 10.1128/JVI.18.2.652-658.1976. View

Vidaver A, Koski R, Van Etten J . Bacteriophage phi6: a Lipid-Containing Virus of Pseudomonas phaseolicola. J Virol. 1973; 11(5):799-805. PMC: 355178. DOI: 10.1128/JVI.11.5.799-805.1973. View

Yang Y, Lang D . Electron microscopy of bacteriophage phi 6 nucleocapsid: three-dimensional image analysis. J Virol. 1984; 51(2):484-8. PMC: 254463. DOI: 10.1128/JVI.51.2.484-488.1984. View

Mindich L . The characterization of a 120 S particle formed during phi 6 infection. Virology. 1980; 103(2):386-91. DOI: 10.1016/0042-6822(80)90197-x. View

Caspar D, Klug A . Physical principles in the construction of regular viruses. Cold Spring Harb Symp Quant Biol. 1962; 27:1-24. DOI: 10.1101/sqb.1962.027.001.005. View

10.

Bamford D, Mindich L . Electron microscopy of cells infected with nonsense mutants of bacteriophage phi 6. Virology. 1980; 107(1):222-8. DOI: 10.1016/0042-6822(80)90287-1. View

11.

Olkkonen V, Bamford D . The nucleocapsid of the lipid-containing double-stranded RNA bacteriophage phi 6 contains a protein skeleton consisting of a single polypeptide species. J Virol. 1987; 61(8):2362-7. PMC: 255646. DOI: 10.1128/JVI.61.8.2362-2367.1987. View

12.

Emori Y, Iba H, Okada Y . Morphogenetic pathway of bacteriophage phi 6. A flow analysis of subviral and viral particles in infected cells. J Mol Biol. 1982; 154(2):287-310. DOI: 10.1016/0022-2836(82)90065-1. View

13.

Sinclair J, Mindich L . RNA synthesis during infection with bacteriophage phi6. Virology. 1976; 75(1):209-17. DOI: 10.1016/0042-6822(76)90019-2. View