Co-Nanoencapsulation of Vitamin D and Curcumin Regulates Inflammation and Purine Metabolism in a Model of Arthritis

Overview

Journal Inflammation

Specialties Allergy & Immunology
Pathology

Date 2019 May 19

PMID 31102126

Citations 7

Authors

Jean Lucas G da Silva

Daniela F Passos

Viviane M Bernardes

Fernanda L Cabral

Paulo G Schimites

Alessandra G Manzoni

Edilene Gadelha de Oliveira

Cristiane de Bona da Silva

Ruy Carlos Ruver Beck

Matheus H Jantsch

Roberto M Maciel

Daniela B R Leal

Affiliations

Soon will be listed here.

Abstract

We analyzed the effects of a nanoencapsulated association of curcumin and vitamin D on purine metabolism enzymes in neutrophils, lymphocytes, and platelets in a model of adjuvant-induced arthritis (AIA) in rats. Following AIA induction, the animals were treated for 15 days with free and nanoencapsulated curcumin (4 mg/kg), nanocapsules of vitamin D (VD3) (15.84 IU/day), a nanoencapsulated combination of curcumin and VD3, vehicle, or blank nanocapsules. The animals were euthanized, and blood was collected to evaluate the activities of E-NTPDase, adenosine deaminase (ADA), and myeloperoxidase (MPO), as well as reactive oxygen species (ROS) levels and biochemical parameters. Also, the liver and kidney were collected for histological analysis. The changes in the activities of purinergic enzymes indicated that inflammation was significantly reverted by vitamin D and curcumin co-nanoencapsulation treatments in the arthritic rats. The reduction of inflammation was confirmed by the reduction in the signs and symptoms of AIA, as well as in MPO activity by all formulations. The treatments with nanocapsules reverted histological alterations in the kidney. Serum parameters were not altered by the induction or treatments. Our results suggest that co-nanoencapsulation of vitamin D and curcumin is an efficient alternative adjuvant treatment for rheumatoid arthritis as it reverts the changes in the purine metabolism and reduces arthritis-associated inflammation.

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