» Articles » PMID: 31086084

Advances in Chemical and Biological Methods to Identify Microorganisms-From Past to Present

Abstract

Fast detection and identification of microorganisms is a challenging and significant feature from industry to medicine. Standard approaches are known to be very time-consuming and labor-intensive (e.g., culture media and biochemical tests). Conversely, screening techniques demand a quick and low-cost grouping of bacterial/fungal isolates and current analysis call for broad reports of microorganisms, involving the application of molecular techniques (e.g., 16S ribosomal RNA gene sequencing based on polymerase chain reaction). The goal of this review is to present the past and the present methods of detection and identification of microorganisms, and to discuss their advantages and their limitations.

Citing Articles

Rapid diagnosis of urinary tract infection with miniaturised point-of-care cultivation on a dipstick.

Iseri E, Jakobsson G, Bertling S, Ozenci V, Ekelund O, van der Wijngaart W Eur J Clin Microbiol Infect Dis. 2025; .

PMID: 40063324 DOI: 10.1007/s10096-025-05088-7.


A systematic study of molecular targets of cannabidiol in Alzheimer's disease.

Patil N, Patil K, Jain M, Mohammed A, Yadav A, Dhanda P J Alzheimers Dis Rep. 2025; 8(1):1339-1360.

PMID: 40034365 PMC: 11863746. DOI: 10.1177/25424823241284464.


Nanoplasmonic SERS on fidget spinner for digital bacterial identification.

Karmacharya M, Michael I, Han J, Clarissa E, Gulenko O, Kumar S Microsyst Nanoeng. 2025; 11(1):38.

PMID: 40025021 PMC: 11873259. DOI: 10.1038/s41378-025-00870-1.


Discriminating bacterial types in oral and maxillofacial space infections (OMSIs) via smelling diagnosis.

Ren R, Jiang X, Zhou S, Li H, Niu Q, Qu D Clin Oral Investig. 2025; 29(3):157.

PMID: 39998688 DOI: 10.1007/s00784-025-06243-2.


biofilms in the establishment of dental caries: a review.

Thayumanavan T, Harish B, Subashkumar R, Shanmugapriya K, Karthik V 3 Biotech. 2025; 15(3):62.

PMID: 39959706 PMC: 11828782. DOI: 10.1007/s13205-025-04227-3.


References
1.
Klingler J, Stowe R, Obenhuber D, Groves T, Mishra S, Pierson D . Evaluation of the Biolog automated microbial identification system. Appl Environ Microbiol. 1992; 58(6):2089-92. PMC: 195731. DOI: 10.1128/aem.58.6.2089-2092.1992. View

2.
Villas-Boas S, Mas S, Akesson M, Smedsgaard J, Nielsen J . Mass spectrometry in metabolome analysis. Mass Spectrom Rev. 2004; 24(5):613-46. DOI: 10.1002/mas.20032. View

3.
Jarvis R, Goodacre R . Discrimination of bacteria using surface-enhanced Raman spectroscopy. Anal Chem. 2003; 76(1):40-7. DOI: 10.1021/ac034689c. View

4.
Reinoso E, Bettera S . Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology. Biochem Mol Biol Educ. 2016; 44(4):391-6. DOI: 10.1002/bmb.20957. View

5.
Agca H, Dalyan Cilo B, Ozmerdiven G, Saglam S, Ener B . [Development of a real-time polymerase chain reaction method for the identification of Candida species]. Mikrobiyol Bul. 2015; 49(1):56-65. DOI: 10.5578/mb.8889. View