Overexpression of T 1 () Induces Extremely Early Flowering in Rice

Overview

Journal Mol Cells

Publisher Elsevier

Specialties Cell Biology
Molecular Biology

Date 2019 May 16

PMID 31085810

Citations 11

Authors

Richa Pasriga

Jinmi Yoon

Lae-Hyeon Cho

Gynheung An

Affiliations

Soon will be listed here.

Abstract

RICE FLOWERING LOCUS T 1 (RFT1) is a major florigen that functions to induce reproductive development in the shoot apical meristem (SAM). To further our study of , we overexpressed the gene and examined the expression patterns of major regulatory genes during floral transition and inflorescence development. Overexpression induced extremely early flowering in the transgenics, and a majority of those calli directly formed spikelets with a few spikelets, thus bypassing normal vegetative development. ()-clade genes , 5, and were highly induced in the -expressing meristems. - was also induced in the meristems. This indicated that RFT1 promotes the expression of major regulatory genes that are important for inflorescence development. RFT1 overexpression also induced SEPALLATA (SEP)-clade genes , , and in the greening calli before floral transition occurred. This suggested their possible roles at the early reproductive stages. We found it interesting that expression of as well as and was strongly increased in the -expressing calli and spikelets. At a low frequency, those calli produced plants with a few leaves that generated a panicle with a small number of spikelets. In the transgenic leaves, the FULclade genes and were induced, but SEP-clade gene expression was not increased. This indicated that , 5, , and act immediately downstream of .

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