Single-atom Nanozymes

Overview

Journal Sci Adv

Specialties Biology
Science

Date 2019 May 7

PMID 31058221

Citations 117

Authors

Liang Huang

Jinxing Chen

Linfeng Gan

Jin Wang

Shaojun Dong

Affiliations

Soon will be listed here.

Abstract

Conventional nanozyme technologies face formidable challenges of intricate size-, composition-, and facet-dependent catalysis and inherently low active site density. We discovered a new class of single-atom nanozymes with atomically dispersed enzyme-like active sites in nanomaterials, which significantly enhanced catalytic performance, and uncovered the underlying mechanism. With oxidase catalysis as a model reaction, experimental studies and theoretical calculations revealed that single-atom nanozymes with carbon nanoframe-confined FeN active centers (FeN SA/CNF) catalytically behaved like the axial ligand-coordinated heme of cytochrome P450. The definite active moieties and crucial synergistic effects endow FeN SA/CNF with a clear electron push-effect mechanism, as well as the highest oxidase-like activity among other nanozymes (the rate constant is 70 times higher than that of commercial Pt/C) and versatile antibacterial applications. These suggest that the single-atom nanozymes have great potential to become the next-generation nanozymes.

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References

Pedone D, Moglianetti M, De Luca E, Bardi G, Pompa P . Platinum nanoparticles in nanobiomedicine. Chem Soc Rev. 2017; 46(16):4951-4975. DOI: 10.1039/c7cs00152e. View

Poulos T . Heme enzyme structure and function. Chem Rev. 2014; 114(7):3919-62. PMC: 3981943. DOI: 10.1021/cr400415k. View

Wei H, Wang E . Nanomaterials with enzyme-like characteristics (nanozymes): next-generation artificial enzymes. Chem Soc Rev. 2013; 42(14):6060-93. DOI: 10.1039/c3cs35486e. View

Chen Q, Liang C, Zhang X, Huang Y . High oxidase-mimic activity of Fe nanoparticles embedded in an N-rich porous carbon and their application for sensing of dopamine. Talanta. 2018; 182:476-483. DOI: 10.1016/j.talanta.2018.02.032. View

Liu W, Zhang L, Liu X, Liu X, Yang X, Miao S . Discriminating Catalytically Active FeN Species of Atomically Dispersed Fe-N-C Catalyst for Selective Oxidation of the C-H Bond. J Am Chem Soc. 2017; 139(31):10790-10798. DOI: 10.1021/jacs.7b05130. View

Reuillard B, Gentil S, Carriere M, Le Goff A, Cosnier S . Biomimetic enzymatic high-potential electrocatalytic reduction of hydrogen peroxide on a functionalized carbon nanotube electrode. Chem Sci. 2017; 6(9):5139-5143. PMC: 5666682. DOI: 10.1039/c5sc01473e. View

Marcinkowski M, Darby M, Liu J, Wimble J, Lucci F, Lee S . Pt/Cu single-atom alloys as coke-resistant catalysts for efficient C-H activation. Nat Chem. 2018; 10(3):325-332. DOI: 10.1038/nchem.2915. View

Yin W, Yu J, Lv F, Yan L, Zheng L, Gu Z . Functionalized Nano-MoS with Peroxidase Catalytic and Near-Infrared Photothermal Activities for Safe and Synergetic Wound Antibacterial Applications. ACS Nano. 2016; 10(12):11000-11011. DOI: 10.1021/acsnano.6b05810. View

Nath I, Chakraborty J, Verpoort F . Metal organic frameworks mimicking natural enzymes: a structural and functional analogy. Chem Soc Rev. 2016; 45(15):4127-70. DOI: 10.1039/c6cs00047a. View

10.

Hou S, Hu X, Wen T, Liu W, Wu X . Core-shell noble metal nanostructures templated by gold nanorods. Adv Mater. 2013; 25(28):3857-62. DOI: 10.1002/adma.201301169. View

11.

Wang Q, Zhang X, Huang L, Zhang Z, Dong S . GOx@ZIF-8(NiPd) Nanoflower: An Artificial Enzyme System for Tandem Catalysis. Angew Chem Int Ed Engl. 2017; 56(50):16082-16085. DOI: 10.1002/anie.201710418. View

12.

Asati A, Santra S, Kaittanis C, Nath S, Perez J . Oxidase-like activity of polymer-coated cerium oxide nanoparticles. Angew Chem Int Ed Engl. 2009; 48(13):2308-12. PMC: 2923475. DOI: 10.1002/anie.200805279. View

13.

Tao Y, Ju E, Ren J, Qu X . Bifunctionalized mesoporous silica-supported gold nanoparticles: intrinsic oxidase and peroxidase catalytic activities for antibacterial applications. Adv Mater. 2015; 27(6):1097-104. DOI: 10.1002/adma.201405105. View

14.

Liu S, Zeng T, Hofmann M, Burcombe E, Wei J, Jiang R . Antibacterial activity of graphite, graphite oxide, graphene oxide, and reduced graphene oxide: membrane and oxidative stress. ACS Nano. 2011; 5(9):6971-80. DOI: 10.1021/nn202451x. View

15.

Natalio F, Andre R, Hartog A, Stoll B, Jochum K, Wever R . Vanadium pentoxide nanoparticles mimic vanadium haloperoxidases and thwart biofilm formation. Nat Nanotechnol. 2012; 7(8):530-5. DOI: 10.1038/nnano.2012.91. View

16.

Xu Z, Qiu Z, Liu Q, Huang Y, Li D, Shen X . Converting organosulfur compounds to inorganic polysulfides against resistant bacterial infections. Nat Commun. 2018; 9(1):3713. PMC: 6137151. DOI: 10.1038/s41467-018-06164-7. View

17.

Liang M, Fan K, Pan Y, Jiang H, Wang F, Yang D . Fe3O4 magnetic nanoparticle peroxidase mimetic-based colorimetric assay for the rapid detection of organophosphorus pesticide and nerve agent. Anal Chem. 2012; 85(1):308-12. DOI: 10.1021/ac302781r. View

18.

Kotov N . Chemistry. Inorganic nanoparticles as protein mimics. Science. 2010; 330(6001):188-9. DOI: 10.1126/science.1190094. View

19.

Jiang B, Duan D, Gao L, Zhou M, Fan K, Tang Y . Standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes. Nat Protoc. 2018; 13(7):1506-1520. DOI: 10.1038/s41596-018-0001-1. View

20.

He W, Kim H, Wamer W, Melka D, Callahan J, Yin J . Photogenerated charge carriers and reactive oxygen species in ZnO/Au hybrid nanostructures with enhanced photocatalytic and antibacterial activity. J Am Chem Soc. 2013; 136(2):750-7. DOI: 10.1021/ja410800y. View