Biosynthetic Reconstitution of Deoxysugar Phosphoramidate Metalloprotease Inhibitors Using an N-P-bond-forming Kinase

Overview

Journal Chem Sci

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2019 May 7

PMID 31057776

Citations 1

Authors

Alexandra Baulig

Irina Helmle

Marius Bader

Felix Wolf

Andreas Kulik

Arwa Al-Dilaimi

Daniel Wibberg

Jorn Kalinowski

Harald Gross

Leonard Kaysser

Affiliations

Soon will be listed here.

Abstract

Phosphoramidon is a potent metalloprotease inhibitor and a widespread tool in cell biology research. It contains a dipeptide backbone that is uniquely linked to a 6-deoxysugar a phosphoramidate bridge. Herein, we report the identification of a gene cluster for the formation of phosphoramidon and its detailed characterization. reconstitution of the biosynthesis established TalE as a phosphoramidate-forming kinase and TalC as the glycosyltransferase which installs the l-rhamnose moiety by phosphoester linkage.

Citing Articles

Advances in the Synthesis and Analysis of Biologically Active Phosphometabolites.

Wohlgemuth R Int J Mol Sci. 2023; 24(4).

PMID: 36834560 PMC: 9961378. DOI: 10.3390/ijms24043150.

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