Molecular Alterations in Dog Pheochromocytomas and Paragangliomas

Overview

Journal Cancers (Basel)

Publisher MDPI

Specialty Oncology

Date 2019 May 5

PMID 31052272

Citations 10

Authors

Esther Korpershoek

Daphne A E R Dieduksman

Guy C M Grinwis

Michael J Day

Claudia E Reusch

Monika Hilbe

Federico Fracassi

Niels M G Krol

Andre G Uitterlinden

Annelies de Klein

Bert Eussen

Hans Stoop

Ronald R de Krijger

Sara Galac

Winand N M Dinjens

Affiliations

Soon will be listed here.

Abstract

8658860258318000Recently, genetic alterations in the genes encoding succinate dehydrogenase subunit B and D ( and ) were identified in pet dogs that presented with spontaneously arising pheochromocytomas (PCC) and paragangliomas (PGL; together PPGL), suggesting dogs might be an interesting comparative model for the study of human PPGL. To study whether canine PPGL resembled human PPGL, we investigated a series of 50 canine PPGLs by immunohistochemistry to determine the expression of synaptophysin (SYP), tyrosine hydroxylase (TH) and succinate dehydrogenase subunit A (SDHA) and B (SDHB). In parallel, 25 canine PPGLs were screened for mutations in and by Sanger sequencing. To detect large chromosomal alterations, single nucleotide polymorphism (SNP) arrays were performed for 11 PPGLs, including cases for which fresh frozen tissue was available. The immunohistochemical markers stained positive in the majority of canine PPGLs. Genetic screening of the canine tumors revealed the previously described variants in four cases; p.Arg38Gln ( = 1) and p.Lys122Arg ( = 3). Furthermore, the SNP arrays revealed large chromosomal alterations of which the loss of chromosome 5, partly homologous to human chromosome 1p and chromosome 11, was the most frequent finding (100% of the six cases with chromosomal alterations). In conclusion, canine and human PPGLs show similar genomic alterations, suggestive of common interspecies PPGL-related pathways.

Citing Articles

Multiple Endocrine Neoplasia with Multiple PGLs in Two Boxer Dogs: Morphological Features, Immunohistochemical Profile and SDHD Gene Mutation Screening.

Semzenisi E, Popa R, Toma C, Balteanu V, Scurtu I, Pop R Vet Sci. 2024; 11(11).

PMID: 39591360 PMC: 11598997. DOI: 10.3390/vetsci11110586.

Metabolomic profiling of pheochromocytomas in dogs: Catecholamine phenotype and tricarboxylic acid cycle metabolites.

van den Berg M, Bechmann N, Kooistra H, van Wolferen M, Timmermans-Sprang E, Peitzsch M J Vet Intern Med. 2024; 38(5):2415-2424.

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Whole transcriptome analysis of canine pheochromocytoma and paraganglioma.

van den Berg M, Kooistra H, Grinwis G, Nicoli S, Golinelli S, Stammeleer L Front Vet Sci. 2023; 10:1155804.

PMID: 37691636 PMC: 10484483. DOI: 10.3389/fvets.2023.1155804.

Model systems in SDHx-related pheochromocytoma/paraganglioma.

Takacs-Vellai K, Farkas Z, osz F, Stewart G Cancer Metastasis Rev. 2021; 40(4):1177-1201.

PMID: 34957538 PMC: 8825606. DOI: 10.1007/s10555-021-10009-z.

Laparoscopic resection of retroperitoneal paraganglioma close to caudal vena cava in a dog.

Park Y, Minamoto T Vet Med Sci. 2021; 7(6):2191-2197.

PMID: 34310847 PMC: 8604107. DOI: 10.1002/vms3.588.

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