Efficient Phytase Secretion and Phytate Degradation by Recombinant JCM 1217

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2019 May 2

PMID 31040837

Citations 6

Authors

Zhongke Sun

Zonghao Yue

Xingdong Yang

Xinqi Hao

Maoping Song

Lili Li

Can Chen

Cuiwei Chu

Chengwei Li

Affiliations

Soon will be listed here.

Abstract

Genetic engineering of probiotics, like bifidobacteria, may improve their microbial cell factory economy. This work designed a novel shuttle plasmid pBPES, which bears exogenous and is stable within JCM 1217. Cloning of three predicted promoters into pBPES proved that all of them drive appA expression in JCM 1217. Transformation of plasmids pBPES-tu and pBPES-groEL into JCM1217 resulted in much more phytase secretion suggests P and P are strong promoters. Further and experiments suggested JCM 1217/pBPES-tu degrades phytate efficiently. In conclusion, the study screened two stronger promoters and constructed a recombinant live probiotic strain for effectively phytase secretion and phytate degradation in gut. The strategy used in the study provided a novel technique for improving the bioaccessibility of phytate and decreasing phosphorus excretion.

Citing Articles

Exploring and Engineering Novel Strong Promoters for High-Level Protein Expression in DB104 through Transcriptome Analysis.

Jun J, Jeong H, Hong K Microorganisms. 2023; 11(12).

PMID: 38138072 PMC: 10745405. DOI: 10.3390/microorganisms11122929.

Wide-genome selection of lactic acid bacteria harboring genes that promote the elimination of antinutritional factors.

Pham H, Kim D, Nguyen T Front Plant Sci. 2023; 14:1145041.

PMID: 37180381 PMC: 10171302. DOI: 10.3389/fpls.2023.1145041.

A Single Strain of (CGMCC 21661) Exhibits Stable Glucose- and Lipid-Lowering Effects by Regulating Gut Microbiota.

Wang Y, Wang X, Xiao X, Yu S, Huang W, Rao B Nutrients. 2023; 15(3).

PMID: 36771383 PMC: 9920280. DOI: 10.3390/nu15030670.

Assessment of the bifidogenic and antibacterial activities of xylooligosaccharide.

Sun Z, Yue Z, Liu E, Li X, Li C Front Nutr. 2022; 9:858949.

PMID: 36091239 PMC: 9453197. DOI: 10.3389/fnut.2022.858949.

A Resource for Cloning and Expression Vectors Designed for Bifidobacteria: Overview of Available Tools and Biotechnological Applications.

Ruiz L, Esteban-Torres M, Van Sinderen D Methods Mol Biol. 2021; 2278:157-182.

PMID: 33649956 DOI: 10.1007/978-1-0716-1274-3_14.

References

Hotz C, Gibson R . Assessment of home-based processing methods to reduce the phytate content and phytate/zinc molar ratio of white maize (Zea mays). J Agric Food Chem. 2001; 49(2):692-8. DOI: 10.1021/jf000462w. View

Vohra A, Satyanarayana T . Phytases: microbial sources, production, purification, and potential biotechnological applications. Crit Rev Biotechnol. 2003; 23(1):29-60. DOI: 10.1080/713609297. View

Tanaka K, Samura K, Kano Y . Structural and functional analysis of pTB6 from Bifidobacterium longum. Biosci Biotechnol Biochem. 2005; 69(2):422-5. DOI: 10.1271/bbb.69.422. View

Kim T, Lei X . An improved method for a rapid determination of phytase activity in animal feed. J Anim Sci. 2005; 83(5):1062-7. DOI: 10.2527/2005.8351062x. View

Klijn A, Moine D, Delley M, Mercenier A, Arigoni F, Pridmore R . Construction of a reporter vector for the analysis of Bifidobacterium longum promoters. Appl Environ Microbiol. 2006; 72(11):7401-5. PMC: 1636199. DOI: 10.1128/AEM.01611-06. View

Haros M, Bielecka M, Honke J, Sanz Y . Myo-inositol hexakisphosphate degradation by Bifidobacterium infantis ATCC 15697. Int J Food Microbiol. 2007; 117(1):76-84. DOI: 10.1016/j.ijfoodmicro.2007.02.021. View

Palacios M, Haros M, Rosell C, Sanz Y . Selection of phytate-degrading human bifidobacteria and application in whole wheat dough fermentation. Food Microbiol. 2007; 25(1):169-76. DOI: 10.1016/j.fm.2007.06.001. View

Shkoporov A, Efimov B, Khokhlova E, Kafarskaia L, Smeianov V . Production of human basic fibroblast growth factor (FGF-2) in Bifidobacterium breve using a series of novel expression/secretion vectors. Biotechnol Lett. 2008; 30(11):1983-8. DOI: 10.1007/s10529-008-9772-8. View

Haros M, Carlsson N, Almgren A, Larsson-Alminger M, Sandberg A, Andlid T . Phytate degradation by human gut isolated Bifidobacterium pseudocatenulatum ATCC27919 and its probiotic potential. Int J Food Microbiol. 2009; 135(1):7-14. DOI: 10.1016/j.ijfoodmicro.2009.07.015. View

10.

Schlemmer U, Frolich W, Prieto R, Grases F . Phytate in foods and significance for humans: food sources, intake, processing, bioavailability, protective role and analysis. Mol Nutr Food Res. 2009; 53 Suppl 2:S330-75. DOI: 10.1002/mnfr.200900099. View

11.

Sanz-Penella J, Tamayo-Ramos J, Sanz Y, Haros M . Phytate reduction in bran-enriched bread by phytase-producing bifidobacteria. J Agric Food Chem. 2009; 57(21):10239-44. DOI: 10.1021/jf9023678. View

12.

Khokhlova E, Efimov B, Kafarskaia L, Shkoporov A . Heterologous expression of secreted biologically active human interleukin-10 in Bifidobacterium breve. Arch Microbiol. 2010; 192(9):769-74. DOI: 10.1007/s00203-010-0606-4. View

13.

Jones C, Tokach M, Dritz S, Ratliff B, Horn N, Goodband R . Efficacy of different commercial phytase enzymes and development of an available phosphorus release curve for Escherichia coli-derived phytases in nursery pigs. J Anim Sci. 2010; 88(11):3631-44. DOI: 10.2527/jas.2010-2936. View

14.

Fukuda S, Toh H, Hase K, Oshima K, Nakanishi Y, Yoshimura K . Bifidobacteria can protect from enteropathogenic infection through production of acetate. Nature. 2011; 469(7331):543-7. DOI: 10.1038/nature09646. View

15.

Liu D, Wang S, Xu B, Guo Y, Zhao J, Liu W . Proteomics analysis of Bifidobacterium longum NCC2705 growing on glucose, fructose, mannose, xylose, ribose, and galactose. Proteomics. 2011; 11(13):2628-38. DOI: 10.1002/pmic.201100035. View

16.

Simons P, Versteegh H, Jongbloed A, Kemme P, Slump P, Bos K . Improvement of phosphorus availability by microbial phytase in broilers and pigs. Br J Nutr. 1990; 64(2):525-40. DOI: 10.1079/bjn19900052. View

17.

Sun Z, Baur A, Zhurina D, Yuan J, Riedel C . Accessing the inaccessible: molecular tools for bifidobacteria. Appl Environ Microbiol. 2012; 78(15):5035-42. PMC: 3416414. DOI: 10.1128/AEM.00551-12. View

18.

Kim Y, Lee J, Kang S, Oh S, Griffiths M . Bifidobacterium spp. influences the production of autoinducer-2 and biofilm formation by Escherichia coli O157:H7. Anaerobe. 2012; 18(5):539-45. DOI: 10.1016/j.anaerobe.2012.08.006. View

19.

Sanz-Penella J, Frontela C, Ros G, Martinez C, Monedero V, Haros M . Application of bifidobacterial phytases in infant cereals: effect on phytate contents and mineral dialyzability. J Agric Food Chem. 2012; 60(47):11787-92. DOI: 10.1021/jf3034013. View

20.

Windhorst S, Lin H, Blechner C, Fanick W, Brandt L, Brehm M . Tumour cells can employ extracellular Ins(1,2,3,4,5,6)P(6) and multiple inositol-polyphosphate phosphatase 1 (MINPP1) dephosphorylation to improve their proliferation. Biochem J. 2012; 450(1):115-25. DOI: 10.1042/BJ20121524. View