Accessing the Inaccessible: Molecular Tools for Bifidobacteria

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2012 May 15

PMID 22582076

Citations 15

Authors

Zhongke Sun

Annika Baur

Daria Zhurina

Jing Yuan

Christian U Riedel

Affiliations

Soon will be listed here.

Abstract

Bifidobacteria are an important group of the human intestinal microbiota that have been shown to exert a number of beneficial probiotic effects on the health status of their host. Due to these effects, bifidobacteria have attracted strong interest in health care and food industries for probiotic applications and several species are listed as so-called "generally recognized as safe" (GRAS) microorganisms. Moreover, recent studies have pointed out their potential as an alternative or supplementary strategy in tumor therapy or as live vaccines. In order to study the mechanisms by which these organisms exert their beneficial effects and to generate recombinant strains that can be used as drug delivery vectors or live vaccines, appropriate molecular tools are indispensable. This review provides an overview of the currently available methods and tools to generate recombinant strains of bifidobacteria. The currently used protocols for transformation of bifidobacteria, as well as replicons, selection markers, and determinants of expression, will be summarized. We will further discuss promoters, terminators, and localization signals that have been used for successful generation of expression vectors.

Citing Articles

Metagenomic Insights into the Taxonomic and Functional Features of Traditional Fermented Milk Products from Russia.

Elcheninov A, Zayulina K, Klyukina A, Kremneva M, Kublanov I, Kochetkova T Microorganisms. 2024; 12(1).

PMID: 38276185 PMC: 10819033. DOI: 10.3390/microorganisms12010016.

Establishing genetic manipulation for novel strains of human gut bacteria.

Sheridan P, Odat M, Scott K Microbiome Res Rep. 2023; 2(1):1.

PMID: 38059211 PMC: 10696588. DOI: 10.20517/mrr.2022.13.

Biotechnological Applications of Probiotics: A Multifarious Weapon to Disease and Metabolic Abnormality.

Singh R, Shadan A, Ma Y Probiotics Antimicrob Proteins. 2022; 14(6):1184-1210.

PMID: 36121610 PMC: 9483357. DOI: 10.1007/s12602-022-09992-8.

Genomic and epigenetic landscapes drive CRISPR-based genome editing in .

Pan M, Morovic W, Hidalgo-Cantabrana C, Roberts A, Walden K, Goh Y Proc Natl Acad Sci U S A. 2022; 119(30):e2205068119.

PMID: 35857876 PMC: 9335239. DOI: 10.1073/pnas.2205068119.

Potential applications of recombinant bifidobacterial proteins in the food industry, biomedicine, process innovation and glycobiology.

Morales-Contreras J, Rodriguez-Perez J, Alvarez-Gonzalez C, Martinez-Lopez M, Juarez-Rojop I, Avila-Fernandez A Food Sci Biotechnol. 2021; 30(10):1277-1291.

PMID: 34721924 PMC: 8519974. DOI: 10.1007/s10068-021-00957-1.

References

Lee J, OSullivan D . Genomic insights into bifidobacteria. Microbiol Mol Biol Rev. 2010; 74(3):378-416. PMC: 2937518. DOI: 10.1128/MMBR.00004-10. View

Baban C, Cronin M, OHanlon D, OSullivan G, Tangney M . Bacteria as vectors for gene therapy of cancer. Bioeng Bugs. 2011; 1(6):385-94. PMC: 3056088. DOI: 10.4161/bbug.1.6.13146. View

Argnani A, Leer R, van Luijk N, Pouwels P . A convenient and reproducible method to genetically transform bacteria of the genus Bifidobacterium. Microbiology (Reading). 1996; 142 ( Pt 1):109-114. DOI: 10.1099/13500872-142-1-109. View

Cronin M, Sleator R, Hill C, Fitzgerald G, Van Sinderen D . Development of a luciferase-based reporter system to monitor Bifidobacterium breve UCC2003 persistence in mice. BMC Microbiol. 2008; 8:161. PMC: 2564955. DOI: 10.1186/1471-2180-8-161. View

Park M, Shin D, Lee K, Ji G . Sequence analysis of plasmid pKJ50 from Bifidobacterium longum. Microbiology (Reading). 1999; 145 ( Pt 3):585-592. DOI: 10.1099/13500872-145-3-585. View

Reyes Escogido M, De Leon Rodriguez A, Barba de la Rosa A . A novel binary expression vector for production of human IL-10 in Escherichia coli and Bifidobacterium longum. Biotechnol Lett. 2007; 29(8):1249-53. DOI: 10.1007/s10529-007-9376-8. View

Yamamoto S, Wada J, Katayama T, Jikimoto T, Nakamura M, Kinoshita S . Genetically modified Bifidobacterium displaying Salmonella-antigen protects mice from lethal challenge of Salmonella Typhimurium in a murine typhoid fever model. Vaccine. 2010; 28(41):6684-91. DOI: 10.1016/j.vaccine.2010.08.007. View

Martinez I, Wallace G, Zhang C, Legge R, Benson A, Carr T . Diet-induced metabolic improvements in a hamster model of hypercholesterolemia are strongly linked to alterations of the gut microbiota. Appl Environ Microbiol. 2009; 75(12):4175-84. PMC: 2698331. DOI: 10.1128/AEM.00380-09. View

Long R, Zeng W, Chen L, Guo J, Lin Y, Huang Q . Bifidobacterium as an oral delivery carrier of oxyntomodulin for obesity therapy: inhibitory effects on food intake and body weight in overweight mice. Int J Obes (Lond). 2010; 34(4):712-9. DOI: 10.1038/ijo.2009.277. View

10.

Taniguchi S, Fujimori M, Sasaki T, Tsutsui H, Shimatani Y, Seki K . Targeting solid tumors with non-pathogenic obligate anaerobic bacteria. Cancer Sci. 2010; 101(9):1925-32. PMC: 11158574. DOI: 10.1111/j.1349-7006.2010.01628.x. View

11.

Chen Q, Fischer J, Benoit V, Dufour N, Youderian P, Leong J . In vitro CpG methylation increases the transformation efficiency of Borrelia burgdorferi strains harboring the endogenous linear plasmid lp56. J Bacteriol. 2008; 190(24):7885-91. PMC: 2593207. DOI: 10.1128/JB.00324-08. View

12.

Cronin M, Morrissey D, Rajendran S, El Mashad S, Van Sinderen D, OSullivan G . Orally administered bifidobacteria as vehicles for delivery of agents to systemic tumors. Mol Ther. 2010; 18(7):1397-407. PMC: 2911250. DOI: 10.1038/mt.2010.59. View

13.

Li X, Fu G, Fan Y, Liu W, Liu X, Wang J . Bifidobacterium adolescentis as a delivery system of endostatin for cancer gene therapy: selective inhibitor of angiogenesis and hypoxic tumor growth. Cancer Gene Ther. 2003; 10(2):105-11. DOI: 10.1038/sj.cgt.7700530. View

14.

Fanning S, Hall L, Cronin M, Zomer A, MacSharry J, Goulding D . Bifidobacterial surface-exopolysaccharide facilitates commensal-host interaction through immune modulation and pathogen protection. Proc Natl Acad Sci U S A. 2012; 109(6):2108-13. PMC: 3277520. DOI: 10.1073/pnas.1115621109. View

15.

Hidaka A, Hamaji Y, Sasaki T, Taniguchi S, Fujimori M . Exogenous cytosine deaminase gene expression in Bifidobacterium breve I-53-8w for tumor-targeting enzyme/prodrug therapy. Biosci Biotechnol Biochem. 2007; 71(12):2921-6. DOI: 10.1271/bbb.70284. View

16.

Nierop Groot M, Nieboer F, Abee T . Enhanced transformation efficiency of recalcitrant Bacillus cereus and Bacillus weihenstephanensis isolates upon in vitro methylation of plasmid DNA. Appl Environ Microbiol. 2008; 74(24):7817-20. PMC: 2607179. DOI: 10.1128/AEM.01932-08. View

17.

Nakamura T, Sasaki T, Fujimori M, Yazawa K, Kano Y, Amano J . Cloned cytosine deaminase gene expression of Bifidobacterium longum and application to enzyme/pro-drug therapy of hypoxic solid tumors. Biosci Biotechnol Biochem. 2003; 66(11):2362-6. DOI: 10.1271/bbb.66.2362. View

18.

Park K, Ji G, Park M, Oh S . Expression of rice glutamate decarboxylase in Bifidobacterium longum enhances gamma-aminobutyric acid production. Biotechnol Lett. 2005; 27(21):1681-4. DOI: 10.1007/s10529-005-2730-9. View

19.

Wu G, Sun Y, Qu W, Huang Y, Lu L, Li L . Application of GFAT as a novel selection marker to mediate gene expression. PLoS One. 2011; 6(2):e17082. PMC: 3038931. DOI: 10.1371/journal.pone.0017082. View

20.

Sgorbati B, Scardovi V, Leblanc D . Plasmids in the genus Bifidobacterium. J Gen Microbiol. 1982; 128(9):2121-31. DOI: 10.1099/00221287-128-9-2121. View