Expression of Cloned Monkey Metallothionein in Escherichia Coli

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1987 Jan 1

PMID 3103531

Citations 13

Authors

Y Murooka

T Nagaoka

Affiliations

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Abstract

Expression vectors were constructed in which a cDNA specifying the monkey kidney metallothionein-II (MT-II) was linked directly to the lambda PR promoter. Enhanced expression of MT-II in Escherichia coli was observed when two initiation signals were tandemly linked to the MT-II gene and the lambda cI+ host cells were induced by nalidixic acid.

Citing Articles

Calcium-assisted sortase A cleavage of SUMOylated metallothionein constructs leads to high-yield production of human MT3.

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Efficient expression of truncated recombinant cadmium-metallothionein gene of a ciliate, Tetrahymena tropicalis lahorensis in Escherichia coli.

Shuja R, Taimuri S, Shakoori F, Shakoori A Mol Biol Rep. 2013; 40(12):7061-8.

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A synthetic cadmium metallothionein gene (PMCd1syn) of Paramecium species: expression, purification and characteristics of metallothionein protein.

Dar S, Shuja R, Shakoori A Mol Biol Rep. 2012; 40(2):983-97.

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Zn- and Cu-thioneins: a functional classification for metallothioneins?.

Palacios O, Atrian S, Capdevila M J Biol Inorg Chem. 2011; 16(7):991-1009.

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Enhanced accumulation of Cd2+ by a Mesorhizobium sp. transformed with a gene from Arabidopsis thaliana coding for phytochelatin synthase.

Sriprang R, Hayashi M, Ono H, Takagi M, Hirata K, Murooka Y Appl Environ Microbiol. 2003; 69(3):1791-6.

PMID: 12620871 PMC: 150072. DOI: 10.1128/AEM.69.3.1791-1796.2003.

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