Efficient Expression of Truncated Recombinant Cadmium-metallothionein Gene of a Ciliate, Tetrahymena Tropicalis Lahorensis in Escherichia Coli

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2013 Nov 8

PMID 24197693

Citations 1

Authors

Rukhsana Nighat Shuja

Shuja Uddin Ahmad Taimuri

Farah Rauf Shakoori

Abdul Rauf Shakoori

Affiliations

Soon will be listed here.

Abstract

Truncated recombinant metallothionein GST-fusion protein has been successfully expressed in Escherichia coli. The previously identified novel Cd-inducible metallothionein (TMCd1) gene from the locally isolated ciliate, Tetrahymena tropicalis lahorensis, was inserted into a pET-41a vector, in frame with a sequence encoding an N-terminal glutathione-S-transferase (GST) tail. Truncated recombinant GST fusion protein has been purified by affinity column chromatography using glutathione sepharose. After enzymatic cleavage of GST tail with enterokinase, the truncated TMCd1 MT shows molecular weight of 11.5 kDa, corresponding to the expected value. This is the first successful report of expression of cadmium metallothionein gene of a ciliate, T. t. lahorensis, reported from this part of the world, in E. coli. This study will further help in characterization of metallothionein protein of this ciliate.

Citing Articles

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PMID: 25798065 PMC: 4366644. DOI: 10.7150/ijbs.11060.

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