The Pan-Plasmidome

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2019 Apr 26

PMID 31019500

Citations 14

Authors

Philip Kelleher

Jennifer Mahony

Francesca Bottacini

Gabriele A Lugli

Marco Ventura

Douwe Van Sinderen

Affiliations

Soon will be listed here.

Abstract

Plasmids are autonomous, self-replicating, extrachromosomal genetic elements that are typically not essential for growth of their host. They may encode metabolic capabilities, which promote the maintenance of these genetic elements, and may allow adaption to specific ecological niches and consequently enhance survival. Genome sequencing of 16 strains revealed the presence of 83 plasmids, including two megaplasmids. The limitations of Pacific Biosciences SMRT sequencing in detecting the total plasmid complement of lactococcal strains is examined, while a combined Illumina/SMRT sequencing approach is proposed to combat these issues. Comparative genome analysis of these plasmid sequences combined with other publicly available plasmid sequence data allowed the definition of the lactococcal plasmidome, and facilitated an investigation into (bio) technologically important plasmid-encoded traits such as conjugation, bacteriocin production, exopolysaccharide (EPS) production, and (bacterio) phage resistance.

Citing Articles

Discovery of antiphage systems in the lactococcal plasmidome.

Grafakou A, Mosterd C, Beck M, Kelleher P, McDonnell B, de Waal P Nucleic Acids Res. 2024; 52(16):9760-9776.

PMID: 39119896 PMC: 11381338. DOI: 10.1093/nar/gkae671.

The lactococcal ICE-ome encodes a repertoire of exchangeable traits with potential industrial relevance.

van der Els S, Boekhorst J, Bron P, Kleerebezem M BMC Genomics. 2024; 25(1):734.

PMID: 39080539 PMC: 11288074. DOI: 10.1186/s12864-024-10646-y.

Plasmid-mediated horizontal gene mobilisation: Insights from two lactococcal conjugative plasmids.

Ortiz Charneco G, McDonnell B, Kelleher P, Buivydas A, Dashko S, de Waal P Microb Biotechnol. 2024; 17(5):e14421.

PMID: 38752994 PMC: 11097999. DOI: 10.1111/1751-7915.14421.

Discovering genetic determinants for cell-to-cell adhesion in two prevalent conjugative lactococcal plasmids.

Ortiz Charneco G, Kelleher P, Buivydas A, de Waal P, van Rijswijck I, van Peij N Curr Res Microb Sci. 2024; 6:100239.

PMID: 38706493 PMC: 11067333. DOI: 10.1016/j.crmicr.2024.100239.

Detection and classification of the integrative conjugative elements of Lactococcus lactis.

van der Els S, Sheombarsing R, van Kempen T, Wels M, Boekhorst J, Bron P BMC Genomics. 2024; 25(1):324.

PMID: 38561675 PMC: 10983677. DOI: 10.1186/s12864-024-10255-9.

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