Natural and Concanavalin A-induced Cytotoxic Activity Towards Continuously Growing B Lymphocytes Derived from Patients with Cutaneous T-cell Lymphoma

Overview

Journal Arch Dermatol Res

Specialty Dermatology

Date 1986 Jan 1

PMID 3101618

Citations 1

Authors

J R Jensen

K Kaltoft

S Bisballe

K Thestrup-Pedersen

Affiliations

Soon will be listed here.

Abstract

Continuously growing T- and B-cell lines were derived from peripheral blood, affected skin, and lymph nodes of patients with mycosis fungoides (MF) and Sézary syndrome (SS). Two lymphoblastoid cell lines (MF-13 and SS-2) were Epstein-Barr virus (EBV)-transformed B cells evaluated by surface immunoglobulin, lack of E-rosette formation, positive EBV nuclear antibody test, and secretion of IgM antibody in a plaque-forming cell assay. Analysis of the natural-killer-cell activity using peripheral blood lymphocytes from patients with MF and healthy control persons towards MF-13 and SS-2 target cells suggested resistance to lysis even in tests supplemented with 1,000 IU/ml human gamma-interferon. However, the cell lines were not per se completely resistant to lysis because lymphocytes from control persons showed significant cytotoxicity in an 18-h assay supplemented with 2 micrograms/ml concanavalin A.

Citing Articles

Highly cytotoxic natural killer cells are associated with poor prognosis in patients with cutaneous T-cell lymphoma.

Mundy-Bosse B, Denlinger N, McLaughlin E, Chakravarti N, Hwang S, Chen L Blood Adv. 2018; 2(15):1818-1827.

PMID: 30054309 PMC: 6093742. DOI: 10.1182/bloodadvances.2018020388.

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