Label-Free Multiphoton Endomicroscopy for Minimally Invasive In Vivo Imaging

Overview

Journal Adv Sci (Weinh)

Specialty Biomedical Engineering

Date 2019 Apr 25

PMID 31016109

Citations 38

Authors

Ashwathama Dilipkumar

Alaa Al-Shemmary

Lucas Kreiss

Kristian Cvecek

Birgitta Carle

Ferdinand Knieling

Jean Gonzales Menezes

Oana-Maria Thoma

Michael Schmidt

Markus F Neurath

Maximilian Waldner

Oliver Friedrich

Sebastian Schurmann

Affiliations

Soon will be listed here.

Abstract

Multiphoton microscopy of cellular autofluorescence and second harmonic generation from collagen facilitates imaging of living cells and tissues without the need for additional fluorescent labels. Here, a compact multiphoton endomicroscope for label-free in vivo imaging in small animals via side-viewing needle objectives is presented. Minimal invasive imaging at cellular resolution is performed in colonoscopy of mice without surgical measures and without fluorescent dyes as a contrast agent. The colon mucosa is imaged repeatedly in the same animal in a mouse model of acute intestinal inflammation to study the process of inflammation at the tissue level within a time period of ten days, demonstrating the capabilities of label-free endomicroscopy for longitudinal studies for the first time.

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