Comparative Analyses of Plasma Amyloid-β Levels in Heterogeneous and Monomerized States by Interdigitated Microelectrode Sensor System

Overview

Journal Sci Adv

Specialties Biology
Science

Date 2019 Apr 20

PMID 31001580

Citations 19

Authors

YoungSoo Kim

Yong Kyoung Yoo

Hye Yun Kim

Jee Hoon Roh

Jinsik Kim

Seungyeop Baek

Jinny Claire Lee

Hye Jin Kim

Myung-Sic Chae

Dahye Jeong

Dongsung Park

Sejin Lee

Hochung Jang

Kyeonghwan Kim

Jeong Hoon Lee

Byung Hyun Byun

Su Yeon Park

Jeong Ho Ha

Kyo Chul Lee

Won Woo Cho

Jae-Seung Kim

Jae-Young Koh

Sang Moo Lim

Kyo Seon Hwang

Affiliations

Soon will be listed here.

Abstract

Detection of amyloid-β (Aβ) aggregates contributes to the diagnosis of Alzheimer disease (AD). Plasma Aβ is deemed a less invasive and more accessible hallmark of AD, as Aβ can penetrate blood-brain barriers. However, correlations between biofluidic Aβ concentrations and AD progression has been tenuous. Here, we introduce a diagnostic technique that compares the heterogeneous and the monomerized states of Aβ in plasma. We used a small molecule, EPPS [4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid], to dissociate aggregated Aβ into monomers to enhance quantification accuracy. Subsequently, Aβ levels of EPPS-treated plasma were compared to those of untreated samples to minimize inter- and intraindividual variations. The interdigitated microelectrode sensor system was used to measure plasma Aβ levels on a scale of 0.1 pg/ml. The implementation of this self-standard blood test resulted in substantial distinctions between patients with AD and individuals with normal cognition (NC), with selectivity and sensitivity over 90%.

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