TET3 Prevents Terminal Differentiation of Adult NSCs by a Non-catalytic Action at Snrpn

Overview

Journal Nat Commun

Specialty Biology

Date 2019 Apr 14

PMID 30979904

Citations 24

Authors

Raquel Montalban-Loro

Anna Lozano-Urena

Mitsuteru Ito

Christel Krueger

Wolf Reik

Anne C Ferguson-Smith

Sacri R Ferron

Affiliations

Soon will be listed here.

Abstract

Ten-eleven-translocation (TET) proteins catalyze DNA hydroxylation, playing an important role in demethylation of DNA in mammals. Remarkably, although hydroxymethylation levels are high in the mouse brain, the potential role of TET proteins in adult neurogenesis is unknown. We show here that a non-catalytic action of TET3 is essentially required for the maintenance of the neural stem cell (NSC) pool in the adult subventricular zone (SVZ) niche by preventing premature differentiation of NSCs into non-neurogenic astrocytes. This occurs through direct binding of TET3 to the paternal transcribed allele of the imprinted gene Small nuclear ribonucleoprotein-associated polypeptide N (Snrpn), contributing to transcriptional repression of the gene. The study also identifies BMP2 as an effector of the astrocytic terminal differentiation mediated by SNRPN. Our work describes a novel mechanism of control of an imprinted gene in the regulation of adult neurogenesis through an unconventional role of TET3.

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