Genetic Construction, Expression, and Melanoma-selective Cytotoxicity of a Diphtheria Toxin-related Alpha-melanocyte-stimulating Hormone Fusion Protein

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1986 Nov 1

PMID 3095831

Citations 28

Authors

J R Murphy

W Bishai

M Borowski

A Miyanohara

J Boyd

S Nagle

Affiliations

Soon will be listed here.

Abstract

The structural gene for diphtheria toxin, tox, has been modified at its Sph I site by the introduction of an oligonucleotide linker encoding a unique Pst I restriction endonuclease site and a synthetic oligonucleotide encoding alpha-melanocyte-stimulating hormone (alpha-MSH). The resulting fusion gene directs the expression of a diphtheria toxin-related alpha-MSH hybrid protein in which the diphtheria toxin receptor-binding domain has been replaced with alpha-MSH sequences. The chimeric toxin has been partially purified from periplasmic extracts of recombinant Escherichia coli K-12 and has been found to be selectively toxic for alpha-MSH receptor-positive human malignant melanoma NEL-M1 cells in vitro.

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