Secretory Expression of Recombinant Diphtheria Toxin Mutants in B. Subtilis

Overview

Journal J Tongji Med Univ

Specialty General Medicine

Date 2003 Aug 27

PMID 12938510

Citations 4

Authors

J Zhou

R Petracca

Affiliations

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Abstract

Three diphtheria toxin (DT) mutants CRM-197, DT-del (148) and DT-E148S-K516A-F530A were cloned in B. Subtilis plasmid PSM604 under the subtilisin signal sequence. The expression was effective in both SMS300 and SMS118, but higher yield of 7.1 mg/L was observed in SMS300 compared with 2.1 mg/L in SMS118. Western blot showed that the recombinant protein could be effectively secreted into the culture medium as a 58 ku peptide, and could be degraded into two peptides of 37 ku and 21 ku.

Citing Articles

Hosts and Heterologous Expression Strategies of Recombinant Toxins for Therapeutic Purposes.

di Leandro L, Colasante M, Pitari G, Ippoliti R Toxins (Basel). 2023; 15(12).

PMID: 38133203 PMC: 10748335. DOI: 10.3390/toxins15120699.

High-Level Production of Soluble Cross-Reacting Material 197 in Cytoplasm Due to Fine Tuning of the Target Gene's mRNA Structure.

Khodak Y, Ryazanova A, Vorobiev I, Kovalchuk A, Ovechko N, Aparin P BioTech (Basel). 2023; 12(1).

PMID: 36648835 PMC: 9844443. DOI: 10.3390/biotech12010009.

Efficient recovery of recombinant CRM197 expressed as inclusion bodies in E.coli.

Park A, Jang S, Kim J, Park Y, Koo B, Lee H PLoS One. 2018; 13(7):e0201060.

PMID: 30021008 PMC: 6051658. DOI: 10.1371/journal.pone.0201060.

Molecular Cloning, Structural Modeling and the Production of Soluble Triple-Mutated Diphtheria Toxoid (K51E/G52E/E148K) Co-expressed with Molecular Chaperones in Recombinant Escherichia coli.

Uthailak N, Mahamad P, Chittavanich P, Yanarojana S, Wijagkanalan W, Petre J Mol Biotechnol. 2017; 59(4-5):117-127.

PMID: 28324209 DOI: 10.1007/s12033-017-0001-3.

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