In-depth Human Plasma Proteome Analysis Captures Tissue Proteins and Transfer of Protein Variants Across the Placenta

Overview

Journal Elife

Specialty Biology

Date 2019 Apr 9

PMID 30958262

Citations 40

Authors

Maria Pernemalm

AnnSofi Sandberg

Yafeng Zhu

Jorrit Boekel

Davide Tamburro

Jochen M Schwenk

Albin Bjork

Marie Wahren-Herlenius

Hanna Amark

Claes-Goran Ostenson

Magnus Westgren

Janne Lehtio

Affiliations

Soon will be listed here.

Abstract

Here, we present a method for in-depth human plasma proteome analysis based on high-resolution isoelectric focusing HiRIEF LC-MS/MS, demonstrating high proteome coverage, reproducibility and the potential for liquid biopsy protein profiling. By integrating genomic sequence information to the MS-based plasma proteome analysis, we enable detection of single amino acid variants and for the first time demonstrate transfer of multiple protein variants between mother and fetus across the placenta. We further show that our method has the ability to detect both low abundance tissue-annotated proteins and phosphorylated proteins in plasma, as well as quantitate differences in plasma proteomes between the mother and the newborn as well as changes related to pregnancy.

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