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Stable Carbon Isotope Ratios for Organic Acids in Commercial Honey Samples

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Journal Food Chem
Date 2019 Apr 9
PMID 30955640
Citations 9
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Abstract

Stable carbon isotope ratios (δC) for glucose, fructose, disaccharides, trisaccharides, and organic acids in 116 commercial honey samples were measured by LC/IRMS. On the basis of EA/IRMS and LC/IRMS authenticity criteria, 39 of the samples were judged to have been adulterated. The δC values for organic acids from pure honey, reported here for the first time, ranged from -33.6 to -26.5‰. The mean ΔδC (glucose-organic acids) value was +3.7 ± 0.9‰. Glucose and organic acid δC values were strongly correlated (R = 0.71, P < 0.001). Gluconic acid, the predominant organic acid in honey, has been reported to be produced via decomposition of glucose by bee glucose-oxidase and certain Gluconobacter spp. This fact was confirmed by isotope analysis.

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