A Novel Evolutionary Conserved Mechanism of RNA Stability Regulates Synexpression of Primordial Germ Cell-specific Genes Prior to the Sex-determination Stage in Medaka

Overview

Journal PLoS Biol

Specialty Biology

Date 2019 Apr 5

PMID 30947255

Citations 6

Authors

Amaury Herpin

Cornelia Schmidt

Susanne Kneitz

Clara Gobe

Martina Regensburger

Aurelie Le Cam

Jerome Montfort

Mateus C Adolfi

Christina Lillesaar

Dagmar Wilhelm

Michael Kraeussling

Brigitte Mourot

Beatrice Porcon

Maelle Pannetier

Eric Pailhoux

Laurence Ettwiller

Dirk Dolle

Yann Guiguen

Manfred Schartl

Affiliations

Soon will be listed here.

Abstract

Dmrt1 is a highly conserved transcription factor, which is critically involved in regulation of gonad development of vertebrates. In medaka, a duplicate of dmrt1-acting as master sex-determining gene-has a tightly timely and spatially controlled gonadal expression pattern. In addition to transcriptional regulation, a sequence motif in the 3' UTR (D3U-box) mediates transcript stability of dmrt1 mRNAs from medaka and other vertebrates. We show here that in medaka, two RNA-binding proteins with antagonizing properties target this D3U-box, promoting either RNA stabilization in germ cells or degradation in the soma. The D3U-box is also conserved in other germ-cell transcripts, making them responsive to the same RNA binding proteins. The evolutionary conservation of the D3U-box motif within dmrt1 genes of metazoans-together with preserved expression patterns of the targeting RNA binding proteins in subsets of germ cells-suggest that this new mechanism for controlling RNA stability is not restricted to fishes but might also apply to other vertebrates.

Citing Articles

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