Leukocyte Cytoskeleton Polarization Is Initiated by Plasma Membrane Curvature from Cell Attachment

Overview

Journal Dev Cell

Publisher Cell Press

Specialties Cell Biology
Reproductive Medicine

Date 2019 Apr 2

PMID 30930167

Citations 20

Authors

Chunguang Ren

Qianying Yuan

Martha Braun

Xia Zhang

Bjorn Petri

Jiasheng Zhang

Dongjoo Kim

Julia Guez-Haddad

Wenzhi Xue

Weijun Pan

Rong Fan

Paul Kubes

Zhaoxia Sun

Yarden Opatowsky

Franck Polleux

Erdem Karatekin

Wenwen Tang

Dianqing Wu

Affiliations

Soon will be listed here.

Abstract

Cell polarization is important for various biological processes. However, its regulation, particularly initiation, is incompletely understood. Here, we investigated mechanisms by which neutrophils break their symmetry and initiate their cytoskeleton polarization from an apolar state in circulation for their extravasation during inflammation. We show here that a local increase in plasma membrane (PM) curvature resulting from cell contact to a surface triggers the initial breakage of the symmetry of an apolar neutrophil and is required for subsequent polarization events induced by chemical stimulation. This local increase in PM curvature recruits SRGAP2 via its F-BAR domain, which in turn activates PI4KA and results in PM PtdIns4P polarization. Polarized PM PtdIns4P is targeted by RPH3A, which directs PIP5K1C90 and subsequent phosphorylated myosin light chain polarization, and this polarization signaling axis regulates neutrophil firm attachment to endothelium. Thus, this study reveals a mechanism for the initiation of cell cytoskeleton polarization.

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