PI4P and PI(4,5)P2 Are Essential but Independent Lipid Determinants of Membrane Identity

Overview

Journal Science

Specialty Science

Date 2012 Jun 23

PMID 22722250

Citations 254

Authors

Gerald R V Hammond

Michael J Fischer

Karen E Anderson

Jon Holdich

Ardita Koteci

Tamas Balla

Robin F Irvine

Affiliations

Soon will be listed here.

Abstract

The quantitatively minor phospholipid phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P(2)] fulfills many cellular functions in the plasma membrane (PM), whereas its synthetic precursor, phosphatidylinositol 4-phosphate (PI4P), has no assigned PM roles apart from PI(4,5)P(2) synthesis. We used a combination of pharmacological and chemical genetic approaches to probe the function of PM PI4P, most of which was not required for the synthesis or functions of PI(4,5)P(2). However, depletion of both lipids was required to prevent PM targeting of proteins that interact with acidic lipids or activation of the transient receptor potential vanilloid 1 cation channel. Therefore, PI4P contributes to the pool of polyanionic lipids that define plasma membrane identity and to some functions previously attributed specifically to PI(4,5)P(2), which may be fulfilled by a more general polyanionic lipid requirement.

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