Identification, Characterization, and Regulatory Mechanisms of a Novel EGR1 Splicing Isoform

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2019 Mar 31

PMID 30925677

Citations 9

Authors

Vincenza Aliperti

Giulia Sgueglia

Francesco Aniello

Emilia Vitale

Laura Fucci

Aldo Donizetti

Affiliations

Soon will be listed here.

Abstract

EGR1 is a transcription factor expressed in many cell types that regulates genes involved in different biological processes including growth, proliferation, and apoptosis. Dysregulation of EGR1 expression has been associated with many pathological conditions such as tumors and brain diseases. Known molecular mechanisms underlying the control of EGR1 function include regulation of transcription, mRNA and protein stability, and post-translational modifications. Here we describe the identification of a splicing isoform for the human gene. The newly identified splicing transcript encodes a shorter protein compared to the canonical EGR1. This isoform lacks a region belonging to the N-terminal activation domain and although it is capable of entering the nucleus, it is unable to activate transcription fully relative to the canonical isoform.

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