Method Validation to Assess in Vivo Cellular and Subcellular Changes in Buccal Mucosa Cells and Saliva Following CBCT Examinations

Overview

Journal Dentomaxillofac Radiol

Publisher Oxford University Press

Date 2019 Mar 27

PMID 30912976

Citations 5

Authors

Niels Belmans

Liese Gilles

Piroska Virag

Mihaela Hedesiu

Benjamin Salmon

Sarah Baatout

Stephane Lucas

Reinhilde Jacobs

Ivo Lambrichts

Marjan Moreels

Affiliations

Soon will be listed here.

Abstract

Objectives: Cone-beam CT (CBCT) is a medical imaging technique used in dental medicine. However, there are no conclusive data available indicating that exposure to X-ray doses used by CBCT are harmless. We aim, for the first time, to characterize the potential age-dependent cellular and subcellular effects related to exposure to CBCT imaging. Current objective is to describe and validate the protocol for characterization of cellular and subcellular changes after diagnostic CBCT.

Methods: Development and validation of a dedicated two-part protocol: 1) assessing DNA double strand breaks (DSBs) in buccal mucosal (BM) cells and 2) oxidative stress measurements in saliva samples. BM cells and saliva samples are collected prior to and 0.5 h after CBCT examination. BM cells are also collected 24 h after CBCT examination. DNA DSBs are monitored in BM cells via immunocytochemical staining for γH2AX and 53BP1. 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG) and total antioxidant capacity are measured in saliva to assess oxidative damage.

Results: Validation experiments show that sufficient BM cells are collected (97.1 ± 1.4 %) and that γH2AX/53BP1 foci can be detected before and after CBCT examination. Collection and analysis of saliva samples, either sham exposed or exposed to IR, show that changes in 8-oxo-dG and total antioxidant capacity can be detected in saliva samples after CBCT examination.

Conclusion: The DIMITRA Research Group presents a two-part protocol to analyze potential age-related biological differences following CBCT examinations. This protocol was validated for collecting BM cells and saliva and for analyzing these samples for DNA DSBs and oxidative stress markers, respectively.

Citing Articles

Genotoxic and Cytotoxic Effects of Cone Beam Computed Tomography and Multidetector Computed Tomography on Exfoliated Buccal Epithelial Cells.

Jahanshahiafshar Z, Ghorbani H, Seyedmajidi M, Nabahati M, Ebrahimnejad Gorji K, Seyedmajidi S Iran J Med Sci. 2023; 48(6):572-581.

PMID: 38094288 PMC: 10715117. DOI: 10.30476/IJMS.2023.96522.2807.

Genotoxicity and cytotoxicity effects of x-rays on the oral mucosa epithelium at different fields of view: A cone beam computed tomography technique.

Faeli Ghadikolaei R, Ghorbani H, Seyedmajidi M, Ebrahimnejad Gorji K, Moudi E, Seyedmajidi S Caspian J Intern Med. 2023; 14(1):121-127.

PMID: 36741479 PMC: 9878913. DOI: 10.22088/cjim.14.1.121.

The usefulness of cone beam computed tomography according to age in cleft lip and palate.

Dinu C, Almasan O, Hedesiu M, Armencea G, Baciut G, Bran S J Med Life. 2022; 15(9):1136-1142.

PMID: 36415532 PMC: 9635226. DOI: 10.25122/jml-2022-0209.

Radiobiological risks following dentomaxillofacial imaging: should we be concerned?.

Belmans N, Oenning A, Salmon B, Baselet B, Tabury K, Lucas S Dentomaxillofac Radiol. 2021; 50(6):20210153.

PMID: 33989056 PMC: 8404518. DOI: 10.1259/dmfr.20210153.

Quantification of DNA Double Strand Breaks and Oxidation Response in Children and Adults Undergoing Dental CBCT Scan.

Belmans N, Gilles L, Vermeesen R, Virag P, Hedesiu M, Salmon B Sci Rep. 2020; 10(1):2113.

PMID: 32034200 PMC: 7005754. DOI: 10.1038/s41598-020-58746-5.

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