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Limited Differential Expression of MiRNAs and Other Small RNAs in LPS-stimulated Human Monocytes

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Journal PLoS One
Date 2019 Mar 26
PMID 30908559
Citations 6
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Abstract

Monocytes are a distinct subset of myeloid cells with diverse functions in early inflammatory immune modulation. While previous studies have surveyed the role of miRNA regulation on different myeloid cell lines and primary cultures, the time-dependent kinetics of inflammatory stimulation on miRNA expression and the relationship between miRNA-to-target RNA expression have not been comprehensively profiled in monocytes. In this study, we use next-generation sequencing and RT-PCR assays to analyze the non-coding small RNA transcriptome of unstimulated and lipopolysaccharide (LPS)-stimulated monocytes at 6 and 24 hours. We identified a miRNA signature consisting of five mature miRNAs (hsa-mir-146a, hsa-mir-155, hsa-mir-9, hsa-mir-147b, and hsa-mir-193a) upregulated by LPS-stimulated monocytes after 6 hours and found that most miRNAs were also upregulated after 24 hours of stimulation. Only one miRNA gene was down-regulated and no other small RNAs were found dysregulated in monocytes after LPS treatment. In addition, novel tRNA-derived fragments were also discovered in monocytes although none showed significant changes upon LPS stimulation. Interrogation of validated miRNA targets by transcriptomic analysis revealed that absolute expression of most miRNA targets implicating in innate immune response decreased over time in LPS-stimulated monocytes although their expression patterns along the treatment were heterogeneous. Our findings reveal a potential role by which selective miRNA upregulation and stable expression of other small RNAs enable monocytes to develop finely tuned cellular responses during acute inflammation.

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References
1.
Bazzoni F, Rossato M, Fabbri M, Gaudiosi D, Mirolo M, Mori L . Induction and regulatory function of miR-9 in human monocytes and neutrophils exposed to proinflammatory signals. Proc Natl Acad Sci U S A. 2009; 106(13):5282-7. PMC: 2664036. DOI: 10.1073/pnas.0810909106. View

2.
Boldin M, Baltimore D . MicroRNAs, new effectors and regulators of NF-κB. Immunol Rev. 2012; 246(1):205-20. DOI: 10.1111/j.1600-065X.2011.01089.x. View

3.
Etzrodt M, Cortez-Retamozo V, Newton A, Zhao J, Ng A, Wildgruber M . Regulation of monocyte functional heterogeneity by miR-146a and Relb. Cell Rep. 2012; 1(4):317-24. PMC: 3334310. DOI: 10.1016/j.celrep.2012.02.009. View

4.
Rusca N, Monticelli S . MiR-146a in Immunity and Disease. Mol Biol Int. 2011; 2011:437301. PMC: 3200075. DOI: 10.4061/2011/437301. View

5.
Liu G, Friggeri A, Yang Y, Park Y, Tsuruta Y, Abraham E . miR-147, a microRNA that is induced upon Toll-like receptor stimulation, regulates murine macrophage inflammatory responses. Proc Natl Acad Sci U S A. 2009; 106(37):15819-24. PMC: 2747202. DOI: 10.1073/pnas.0901216106. View