Antiviral Peptides Targeting the West Nile Virus Envelope Protein

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2006 Dec 8

PMID 17151121

Citations 54

Authors

Fengwei Bai

Terrence Town

Deepti Pradhan

Jonathan Cox

Ashish

Michel Ledizet

John F Anderson

Richard A Flavell

Joanna K Krueger

Raymond A Koski

Erol Fikrig

Affiliations

Soon will be listed here.

Abstract

West Nile virus (WNV) can cause fatal murine and human encephalitis. The viral envelope protein interacts with host cells. A murine brain cDNA phage display library was therefore probed with WNV envelope protein, resulting in the identification of several adherent peptides. Of these, peptide 1 prevented WNV infection in vitro with a 50% inhibition concentration of 67 muM and also inhibited infection of a related flavivirus, dengue virus. Peptide 9, a derivative of peptide 1, was a particularly potent inhibitor of WNV in vitro, with a 50% inhibition concentration of 2.6 muM. Moreover, mice challenged with WNV that had been incubated with peptide 9 had reduced viremia and fatality compared with control animals. Peptide 9 penetrated the murine blood-brain barrier and was found in the brain parenchyma, implying that it may have antiviral activity in the central nervous system. These short peptides serve as the basis for developing new therapeutics for West Nile encephalitis and, potentially, other flaviviruses.

Citing Articles

Analysis, Modeling, and Target-Specific Predictions of Linear Peptides Inhibiting Virus Entry.

Vishnepolsky B, Grigolava M, Gabrielian A, Rosenthal A, Hurt D, Tartakovsky M ACS Omega. 2023; 8(48):46218-46226.

PMID: 38075802 PMC: 10701718. DOI: 10.1021/acsomega.3c07521.

The Anti-Dengue Virus Peptide DV2 Inhibits Zika Virus Both In Vitro and In Vivo.

de Castro-Amarante M, Pereira S, Pereira L, Santos L, Venceslau-Carvalho A, Martins E Viruses. 2023; 15(4).

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Molecular Mechanisms of Antiviral Agents against Dengue Virus.

Lee M, Wu Y, Poh C Viruses. 2023; 15(3).

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Plantaricin NC8 αβ rapidly and efficiently inhibits flaviviruses and SARS-CoV-2 by disrupting their envelopes.

Omer A, Hinkula J, Tran P, Melik W, Zattarin E, Aili D PLoS One. 2022; 17(11):e0278419.

PMID: 36449554 PMC: 9710782. DOI: 10.1371/journal.pone.0278419.

Introduction to West Nile Virus.

Karim S, Bai F Methods Mol Biol. 2022; 2585:1-7.

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