Antioxidative and Cardioprotective Effects of Bee Pollen Extract on Isoprenaline-Induced Myocardial Infarction in Rats

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2019 Mar 23

PMID 30897711

Citations 23

Authors

Zhenhuang Shen

Qianqian Geng

Haibo Huang

Hong Yao

Tianyu Du

LiFu Chen

Zhenhong Wu

Xiaoqing Miao

Peiying Shi

Affiliations

Soon will be listed here.

Abstract

Oxidative stress plays an important role in the pathogenesis of myocardial infarction (MI). bee pollen extract (SCBPE) possesses powerful antioxidant capacity. This study aimed to further explore the antioxidative and cardioprotective effects of SCBPE on acute MI induced by isoprenaline (ISO) in rats. The rats were intragastrically administrated with SCBPE (600, 1200, or 1800 mg/kg/day) and Compound Danshen dropping pills (270 mg/kg/day) for 30 days, then subcutaneously injected with ISO (65 mg/kg/day) on the 29th and 30th day. Compared with the model group, pretreatment with middle and high doses of SCBPE significantly reduced serum aspartate transaminase, lactate dehydrogenase, and creatine kinase activities and increased myocardial superoxide dismutase, glutathione peroxidase, and catalase activities. The histopathologic aspects showed that pathological heart change was found in the model group and reduced to varying degrees in the SCBPE groups. Moreover, the protein expression of nuclear factor-erythroid 2-related factor 2 (Nrf-2), heme oxygenase-1 (HO-1), and Bcl2 in the heart increased in the SCBPE groups, while that of Bax decreased compared to the model group. Besides this, uridine was isolated from bee pollen for the first time. This study could provide a scientific basis for using bee pollen as a functional food for the prevention of MI.

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References

Benzie I, Strain J . The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem. 1996; 239(1):70-6. DOI: 10.1006/abio.1996.0292. View

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M . Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999; 26(9-10):1231-7. DOI: 10.1016/s0891-5849(98)00315-3. View

Locatelli M, Macchione N, Ferrante C, Chiavaroli A, Recinella L, Carradori S . Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS. Molecules. 2018; 23(5). PMC: 6100541. DOI: 10.3390/molecules23051145. View

Anandan R, Ganesan B, Obulesu T, Mathew S, Kumar R, Lakshmanan P . Dietary chitosan supplementation attenuates isoprenaline-induced oxidative stress in rat myocardium. Int J Biol Macromol. 2012; 51(5):783-7. DOI: 10.1016/j.ijbiomac.2012.07.016. View

Zhang H, Chen R, Sun G, Yang L, Zhu Y, Xu X . Protective effects of total flavonoids from Clinopodium chinense (Benth.) O. Ktze on myocardial injury in vivo and in vitro via regulation of Akt/Nrf2/HO-1 pathway. Phytomedicine. 2018; 40:88-97. DOI: 10.1016/j.phymed.2018.01.004. View

Hegstad A, Ytrehus K, Myklebust R, Jorgensen L . Ultrastructural changes in the myocardial myocytic mitochondria: crucial step in the development of oxygen radical-induced damage in isolated rat hearts?. Basic Res Cardiol. 1994; 89(2):128-38. DOI: 10.1007/BF00788732. View

El-Gohary O, Allam M . Effect of vitamin D on isoprenaline-induced myocardial infarction in rats: possible role of peroxisome proliferator-activated receptor-γ. Can J Physiol Pharmacol. 2017; 95(6):641-646. DOI: 10.1139/cjpp-2016-0150. View

Cory S, Adams J . The Bcl2 family: regulators of the cellular life-or-death switch. Nat Rev Cancer. 2002; 2(9):647-56. DOI: 10.1038/nrc883. View

Al-Taweel A, Raish M, Perveen S, Fawzy G, Ahmad A, Ansari M . Nepeta deflersiana attenuates isoproterenol-induced myocardial injuries in rats: Possible involvement of oxidative stress, apoptosis, inflammation through nuclear factor (NF)-κB downregulation. Phytomedicine. 2017; 34:67-75. DOI: 10.1016/j.phymed.2017.08.003. View

10.

Bagatini M, Martins C, Battisti V, Gasparetto D, da Rosa C, Spanevello R . Oxidative stress versus antioxidant defenses in patients with acute myocardial infarction. Heart Vessels. 2010; 26(1):55-63. DOI: 10.1007/s00380-010-0029-9. View

11.

Lakkisto P, Palojoki E, Backlund T, Saraste A, Tikkanen I, Pulkki K . Expression of heme oxygenase-1 in response to myocardial infarction in rats. J Mol Cell Cardiol. 2002; 34(10):1357-65. DOI: 10.1006/jmcc.2002.2094. View

12.

Karthikeyan K, Bai B, Niranjali Devaraj S . Cardioprotective effect of grape seed proanthocyanidins on isoproterenol-induced myocardial injury in rats. Int J Cardiol. 2006; 115(3):326-33. DOI: 10.1016/j.ijcard.2006.03.016. View

13.

Selvaraj P, Pugalendi K . Hesperidin, a flavanone glycoside, on lipid peroxidation and antioxidant status in experimental myocardial ischemic rats. Redox Rep. 2010; 15(5):217-23. PMC: 7067333. DOI: 10.1179/135100010X12826446921509. View

14.

Novo G, Cappello F, Rizzo M, Fazio G, Zambuto S, Tortorici E . Hsp60 and heme oxygenase-1 (Hsp32) in acute myocardial infarction. Transl Res. 2011; 157(5):285-92. DOI: 10.1016/j.trsl.2011.01.003. View

15.

Suchal K, Bhatia J, Malik S, Malhotra R, Gamad N, Goyal S . Seabuckthorn Pulp Oil Protects against Myocardial Ischemia-Reperfusion Injury in Rats through Activation of Akt/eNOS. Front Pharmacol. 2016; 7:155. PMC: 4925700. DOI: 10.3389/fphar.2016.00155. View

16.

Liu D, Chen L, Zhao J, Cui K . Cardioprotection activity and mechanism of Astragalus polysaccharide in vivo and in vitro. Int J Biol Macromol. 2018; 111:947-952. DOI: 10.1016/j.ijbiomac.2018.01.048. View

17.

Balogun E, Hoque M, Gong P, Killeen E, Green C, Foresti R . Curcumin activates the haem oxygenase-1 gene via regulation of Nrf2 and the antioxidant-responsive element. Biochem J. 2003; 371(Pt 3):887-95. PMC: 1223348. DOI: 10.1042/BJ20021619. View

18.

Misra M, Sarwat M, Bhakuni P, Tuteja R, Tuteja N . Oxidative stress and ischemic myocardial syndromes. Med Sci Monit. 2009; 15(10):RA209-219. View

19.

Ganesan B, Buddhan S, Anandan R, Sivakumar R, AnbinEzhilan R . Antioxidant defense of betaine against isoprenaline-induced myocardial infarction in rats. Mol Biol Rep. 2009; 37(3):1319-27. DOI: 10.1007/s11033-009-9508-4. View

20.

Maruyama H, Sakamoto T, Araki Y, Hara H . Anti-inflammatory effect of bee pollen ethanol extract from Cistus sp. of Spanish on carrageenan-induced rat hind paw edema. BMC Complement Altern Med. 2010; 10:30. PMC: 2906419. DOI: 10.1186/1472-6882-10-30. View