Acaricidal Mechanism of Scopoletin Against

Overview

Journal Front Physiol

Date 2019 Mar 22

PMID 30894818

Citations 4

Authors

Hong Zhou

Yong-Qiang Zhang

Ting Lai

Xue-Jiao Liu

Fu-You Guo

Tao Guo

Wei Ding

Affiliations

Soon will be listed here.

Abstract

Scopoletin is a promising acaricidal botanical natural compound against , and its acaricidal mechanism maybe involve calcium overload according to our previous study. To seek potential candidate target genes of calcium overload induced by scopoletin in , RNA-seq was utilized to detect changes in transcription levels. 24 and 48 h after treatment, 70 and 102 differentially expressed genes were obtained, respectively. Target genes included 3 signal transduction genes, 4 cell apoptosis genes, 4 energy metabolism genes, and 2 transcription factor genes. The role of 3 calcium signaling pathway-related genes, namely, G-protein-coupled neuropeptide receptor, Bcl-2 protein and guanylate kinase (designated , , and , respectively) in the calcium overload were investigated in this study. RT-qPCR detection showed that scopoletin treatment upregulated the expression level of and downregulated the expression level of and . The result of RNAi indicated that downregulation of decreased susceptibility to scopoletin, and downregulation of and enhanced susceptibility to scopoletin. Functional expression in Chinese hamster ovary cells showed that scopoletin induced a significant increase in intracellular free calcium [Ca]i levels by activating These results demonstrated that the acaricidal mechanism of scopoletin was via disrupting intracellular Ca homeostasis and calcium signaling pathway mediated by GPCR, BAG, and GUK.

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References

Jang D, Park E, Kang Y, Su B, Hawthorne M, Vigo J . Compounds obtained from sida acuta with the potential to induce quinone reductase and to inhibit 7,12-dimethylbenz[a]anthracene-induced preneoplastic lesions in a mouse mammary organ culture model. Arch Pharm Res. 2003; 26(8):585-90. DOI: 10.1007/BF02976704. View

Grbic M, Van Leeuwen T, Clark R, Rombauts S, Rouze P, Grbic V . The genome of Tetranychus urticae reveals herbivorous pest adaptations. Nature. 2011; 479(7374):487-92. PMC: 4856440. DOI: 10.1038/nature10640. View

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S . MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011; 28(10):2731-9. PMC: 3203626. DOI: 10.1093/molbev/msr121. View

Shibasaki F, Kondo E, Akagi T, McKeon F . Suppression of signalling through transcription factor NF-AT by interactions between calcineurin and Bcl-2. Nature. 1997; 386(6626):728-31. DOI: 10.1038/386728a0. View

Shaw C, Chen C, Hsu C, Chen C, Tsai Y . Antioxidant properties of scopoletin isolated from Sinomonium acutum. Phytother Res. 2003; 17(7):823-5. DOI: 10.1002/ptr.1170. View

Cassady J, OJIMA N, Chang C, McLaughlin J . An investigation of the antitumor activity of Micromelum integerrimum (Rutaceae). J Nat Prod. 1979; 42(3):274-8. DOI: 10.1021/np50003a005. View

Liu X, Zhang L, Fu X, Chen K, Qian B . Effect of scopoletin on PC3 cell proliferation and apoptosis. Acta Pharmacol Sin. 2001; 22(10):929-33. View

Pinton P, Ferrari D, Magalhaes P, Schulze-Osthoff K, Di Virgilio F, Pozzan T . Reduced loading of intracellular Ca(2+) stores and downregulation of capacitative Ca(2+) influx in Bcl-2-overexpressing cells. J Cell Biol. 2000; 148(5):857-62. PMC: 2174537. DOI: 10.1083/jcb.148.5.857. View

Nicotera P, Orrenius S . The role of calcium in apoptosis. Cell Calcium. 1998; 23(2-3):173-80. DOI: 10.1016/s0143-4160(98)90116-6. View

10.

Pan R, Gao X, Lu D, Xu X, Xia Y, Dai Y . Prevention of FGF-2-induced angiogenesis by scopoletin, a coumarin compound isolated from Erycibe obtusifolia Benth, and its mechanism of action. Int Immunopharmacol. 2011; 11(12):2007-16. DOI: 10.1016/j.intimp.2011.08.012. View

11.

Manuele M, Ferraro G, Arcos M, Lopez P, Cremaschi G, Anesini C . Comparative immunomodulatory effect of scopoletin on tumoral and normal lymphocytes. Life Sci. 2006; 79(21):2043-8. DOI: 10.1016/j.lfs.2006.06.045. View

12.

Zhu L, Ling S, Yu X, Venkatesh L, Subramanian T, Chinnadurai G . Modulation of mitochondrial Ca(2+) homeostasis by Bcl-2. J Biol Chem. 1999; 274(47):33267-73. DOI: 10.1074/jbc.274.47.33267. View

13.

Zhang Y, Zhang Z, Yutaka S, Liu Q, Ji J . [On the causes of mite pest outbreaks in mono- and poly-cultured moso bamboo forests]. Ying Yong Sheng Tai Xue Bao. 2004; 15(7):1161-5. View

14.

Sun W, Jin Y, He L, Lu W, Li M . Suitable reference gene selection for different strains and developmental stages of the carmine spider mite, Tetranychus cinnabarinus, using quantitative real-time PCR. J Insect Sci. 2011; 10:208. PMC: 3029232. DOI: 10.1673/031.010.20801. View

15.

Carpinella M, Ferrayoli C, Palacios S . Antifungal synergistic effect of scopoletin, a hydroxycoumarin isolated from Melia azedarach L. fruits. J Agric Food Chem. 2005; 53(8):2922-7. DOI: 10.1021/jf0482461. View

16.

Hoffenberg S, Liu X, Nikolova L, Hall H, Dai W, Baughn R . A novel membrane-anchored Rab5 interacting protein required for homotypic endosome fusion. J Biol Chem. 2000; 275(32):24661-9. DOI: 10.1074/jbc.M909600199. View

17.

Casey P, GILMAN A . G protein involvement in receptor-effector coupling. J Biol Chem. 1988; 263(6):2577-80. View

18.

Shaw J, Hamblin M, Kelly D . Purification, characterization and nucleotide sequence of the periplasmic C4-dicarboxylate-binding protein (DctP) from Rhodobacter capsulatus. Mol Microbiol. 1991; 5(12):3055-62. DOI: 10.1111/j.1365-2958.1991.tb01865.x. View

19.

Williams M, Cassady J . Potential antitumor agents: a cytotoxic cardenolide from Coronilla varia L. J Pharm Sci. 1976; 65(6):912-4. DOI: 10.1002/jps.2600650628. View

20.

von der Lehr N, Johansson S, Larsson L . Implication of the ubiquitin/proteasome system in Myc-regulated transcription. Cell Cycle. 2003; 2(5):403-7. View