The Influence of Heat-Killed BGPAS1-3 on the Tight Junction Protein Expression and Immune Function in Differentiated Caco-2 Cells Infected With ATCC 19111

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2019 Mar 21

PMID 30891021

Citations 22

Authors

Nikola Popovic

Jelena Djokic

Emilija Brdaric

Miroslav Dinic

Amarela Terzic-Vidojevic

Natasa Golic

Katarina Veljovic

Affiliations

Soon will be listed here.

Abstract

, the common foodborne pathogenic bacteria species, compromises the intestinal epithelial barrier, leading to development of the listeriosis, a severe disease especially among immunocompromised individuals. infection usually requires antibiotic treatment, however, excessive use of antibiotics promotes emergence of antibiotic resistance and the destruction of gut microbiota. Probiotics, including lactic acid bacteria (LAB), have been repeatedly proven as an alternative approach for the treatment of various infections. We have analyzed the potential of BGPAS1-3, a dairy isolate exhibiting strong direct antilisterial effect, to modulate the response of differentiated Caco-2 intestinal epithelial cells to ATCC 19111 infection. We showed that the molecule with antilisterial effect is a bacterial cell-wall protein that is highly resistant to the high-temperature treatment. When we tested the antilisterial potential of heat-killed BGPAS1-3, we found that it could prevent tight junction disruption in differentiated Caco-2 monolayer infected with ATCC 19111, induce antilisterial host response mechanisms, and stimulate the production of protective TGF-β in intestinal epithelial cells. We also showed that the modulation of MyD88 dependent TLR2 and TLR4 pathways by BGPAS1-3 are involved in host response against ATCC 19111. Since heat-killed BGPAS1-3 possess strong antilisterial effects, such postbiotic could be used as a controllable and safe therapeutic.

Citing Articles

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Insight into the Probiogenomic Potential of BGPAS1-3 and Application of a Potent Thermostable Bacteriocin.

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Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing BGZLM1-5 in the Reduction in the Abundance of ATCC19111 in a Milk Model.

Popovic N, Stevanovic D, Radojevic D, Veljovic K, dokic J, Golic N Microorganisms. 2023; 11(12).

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