Tetraspanin 1 As a Mediator of Fibrosis Inhibits EMT Process and Smad2/3 and Beta-catenin Pathway in Human Pulmonary Fibrosis

Overview

Journal J Cell Mol Med

Specialties Cell Biology
Molecular Biology

Date 2019 Mar 15

PMID 30869194

Citations 12

Authors

Gang Liu

Yahong Wang

Lawei Yang

Baoan Zou

Shenglan Gao

Zeqing Song

Ziying Lin

Affiliations

Soon will be listed here.

Abstract

Tetraspanin 1(TSPAN1) as a clinically relevant gene target in cancer has been studied, but there is no direct in vivo or vitro evidence for pulmonary fibrosis (PF). Using reanalysing Gene Expression Omnibus data, here, we show for the first time that TSPAN1 was markedly down-regulated in lung tissue of patient with idiopathic PF (IPF) and verified the reduced protein expression of TSPAN1 in lung tissue samples of patient with IPF and bleomycin-induced PF mice. The expression of TSPAN1 was decreased and associated with transforming growth factor-β1 (TGF-β )-induced molecular characteristics of epithelial-to-mesenchymal transition (EMT) in alveolar epithelial cells (AECs). Silencing TSPAN1 promoted cell migration, and the expression of alpha-smooth muscle actin, vimentin and E-cadherin in AECs with TGF-β treatment, while exogenous TSPAN1 has the converse effects. Moreover, silencing TSPAN1 promotes the phosphorylation of Smad2/3 and stabilizes beta-catenin protein, however, overexpressed TSPAN1 impeded TGF-β -induced activation of Smad2/3 and beta-catenin pathway in AECs. Together, our study implicates TSPAN1 as a key regulator in the process of EMT in AECs of IPF.

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