Comparison of MYCOPLASMELISA with Complement Fixation Test for Measurement of Antibodies to Mycoplasma Pneumoniae

The MYCOPLASMELISA test kit was compared with the complement fixation test for detection of Mycoplasma pneumoniae antibody in single sera and evaluation of acute- and convalescent-phase serum pairs for significant rises in antibody titers. Agreement between the two assays was 96.6% (85 of 88) for antibody detection and 93.9% (92 of 98) for evaluation of paired sera. Sensitivity and specificity of MYCOPLASMELISA, relative to complement fixation, for the serodiagnosis of recent M. pneumoniae infection was 92.2% (59 of 64) and 95% (19 of 20), respectively.