Endometriotic Mesenchymal Stem Cells Epigenetic Pathogenesis: Deregulation of , , and in A Functional Imbalanced Epigenetic Disease

Overview

Journal Cell J

Specialty Cell Biology

Date 2019 Mar 3

PMID 30825291

Citations 13

Authors

Parisa Mashayekhi

Mehrdad Noruzinia

Sirous Zeinali

Sepideh Khodaverdi

Affiliations

Soon will be listed here.

Abstract

Objective: Stem cell issue is a strong theory in endometriosis pathogenesis. It seems that endometriotic mesenchymal stem cells (MSCs) show different characteristics compared to the normal MSCs. Determined high proliferation and low differentiation/decidualization potential of endometriotic MSCs could be accompanied by their microRNAs deregulation influencing their fate and function. In this study for the first time, we evaluated the expression of , , and in endometriotic compared to non-endometriotic MSCs. These microRNAs are involved in biological pathways related to proliferation and differentiation of stem cells. Their aberrant expressions can disturb the proliferation/ differentiation balance in stem cells, altering their function and causing various diseases, like endometriosis.

Materials And Methods: In this experimental study, MSCs were isolated from three endometriotic and three nonendometriotic eutopic endometrium, followed by their characterization and culture. Expression of , , and was ultimately analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR).

Results: We found that the expression of was up-regulated (P<0.0001) whereas the expression of and was down-regulated (P<0.0001) in endometriotic MSCs in comparison with non-endometriotic normal controls.

Conclusion: Proliferation and differentiation are important dynamic balanced biological processes, while in equillibrium, they determine a healthy stem cell fate. It seems that they are deregulated in endometriotic MSCs and change their function. , , and are deregulated during endometriosis and they have pivotal roles in the modulating proliferation and differentiation of stem cells. We found up-regulation of and down-regulation of and in endometriotic MSCs. These changes can increase self-renewal and migration, while decreasing differentiation of endometriotic MSCs. Our achievements emphasize previous findings on the importance of proliferation/ differentiation balance in MSCs and clarify the role of microRNAs as main players in faulty endometriotic stem cells development.

Citing Articles

Expression of and in Different Forms of Endometriosis.

Ntzeros K, Voros C, Mavrogianni D, Kathopoulis N, Kypriotis K, Varthaliti A Biomedicines. 2025; 13(2).

PMID: 40002936 PMC: 11852903. DOI: 10.3390/biomedicines13020524.

Metformin as a potential agent for modulating the faulty endometriotic mesenchymal stem cells: A case-control study.

Mashayekhi P, Noruzinia M, Khodaverdi S Int J Reprod Biomed. 2022; 20(10):861-872.

PMID: 36381355 PMC: 9644643. DOI: 10.18502/ijrm.v20i10.12270.

Overexpression of miR-200b-3p in Menstrual Blood-Derived Mesenchymal Stem Cells from Endometriosis Women.

de Oliveira R, Buono F, Cressoni A, Penariol L, Padovan C, Tozetti P Reprod Sci. 2022; 29(3):734-742.

PMID: 35075610 DOI: 10.1007/s43032-022-00860-y.

Knockdown hsa_circ_0063526 inhibits endometriosis progression via regulating the miR-141-5p / EMT axis and downregulating estrogen receptors.

Wei Z, Hu Y, He X, Zhang M, Zhang X, Wang Y Aging (Albany NY). 2021; 13(24):26095-26117.

PMID: 34967761 PMC: 8751610. DOI: 10.18632/aging.203799.

MiR-200c-3p maintains stemness and proliferative potential in adipose-derived stem cells by counteracting senescence mechanisms.

Anastasiadou E, Ceccarelli S, Messina E, Gerini G, Megiorni F, Pontecorvi P PLoS One. 2021; 16(9):e0257070.

PMID: 34534238 PMC: 8448302. DOI: 10.1371/journal.pone.0257070.

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