Inhibitory Effects of Polysorbate 80 on MRSA Biofilm Formed on Different Substrates Including Dermal Tissue

Overview

Journal Sci Rep

Specialty Science

Date 2019 Mar 1

PMID 30816342

Citations 22

Authors

Yutaka Ueda

Kota Mashima

Motoyasu Miyazaki

Shuuji Hara

Tohru Takata

Hidetoshi Kamimura

Satoshi Takagi

Shiro Jimi

Affiliations

Soon will be listed here.

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) forms biofilms on necrotic tissues and medical devices, and causes persistent infections. Surfactants act on biofilms, but their mode of action is still unknown. If used in the clinic, cytotoxicity in tissues should be minimized. In this study, we investigated the inhibitory effect of four different surfactants on MRSA biofilm formation, and found that a nonionic surfactant, polysorbate 80 (PS80), was the most suitable. The biofilm inhibitory effects resulted from the inhibition of bacterial adhesion to substrates rather than biofilm disruption, and the effective dose was less cytotoxic for 3T3 fibroblasts. However, the effects were substrate-dependent: positive for plastic, silicon, and dermal tissues, but negative for stainless-steel. These results indicate that PS80 is effective for prevention of biofilms formed by MRSA on tissues and foreign bodies. Therefore, PS80 could be used in medical practice as a washing solution for wounds and/or pretreatment of indwelling catheters.

Citing Articles

Naringenin-Loaded Solid Lipid Nanoparticles: Physical-Chemical Characterization and In Vitro Antibacterial Activity.

De Gaetano F, Caridi F, Totaro N, Celesti C, Venuti V, Ginestra G Pharmaceuticals (Basel). 2025; 18(2).

PMID: 40006044 PMC: 11859375. DOI: 10.3390/ph18020232.

Equine bone marrow-derived mesenchymal stromal cells reduce established S. aureus and E. coli biofilm matrix in vitro.

Khatibzadeh S, Dahlgren L, Caswell C, Ducker W, Werre S, Bogers S PLoS One. 2024; 19(10):e0312917.

PMID: 39480794 PMC: 11527187. DOI: 10.1371/journal.pone.0312917.

In Vitro Antibacterial and Antibiofilm Activities of L. Ethanolic Leaf Extract on ATCC 29213.

Lao R, Yabes A, Tobias-Altura M, Panganiban L, Makalinao I Acta Med Philipp. 2024; 57(12):53-60.

PMID: 39429760 PMC: 11484527. DOI: 10.47895/amp.vi0.6412.

Investigating the Bactericidal Activity of an Ocular Solution Containing EDTA, Tris, and Polysorbate 80 and Its Impact on the In Vitro Efficacy of Neomycin Sulfate against : A Preliminary Study.

Amiriantz S, Hoummady S, Jarousse E, Roudeix S, Philippon T Antibiotics (Basel). 2024; 13(7).

PMID: 39061293 PMC: 11273460. DOI: 10.3390/antibiotics13070611.

Surfactants' Interplay with Biofilm Development in and .

Aonofriesei F Pharmaceutics. 2024; 16(5).

PMID: 38794319 PMC: 11125353. DOI: 10.3390/pharmaceutics16050657.

References

Garcia L, Lemaire S, Kahl B, Becker K, Proctor R, Denis O . Antibiotic activity against small-colony variants of Staphylococcus aureus: review of in vitro, animal and clinical data. J Antimicrob Chemother. 2013; 68(7):1455-64. DOI: 10.1093/jac/dkt072. View

Evans R, Holmes C . Effect of vancomycin hydrochloride on Staphylococcus epidermidis biofilm associated with silicone elastomer. Antimicrob Agents Chemother. 1987; 31(6):889-94. PMC: 284205. DOI: 10.1128/AAC.31.6.889. View

Kline K, Falker S, Dahlberg S, Normark S, Henriques-Normark B . Bacterial adhesins in host-microbe interactions. Cell Host Microbe. 2009; 5(6):580-92. DOI: 10.1016/j.chom.2009.05.011. View

Bensing B, Lopez J, Sullam P . The Streptococcus gordonii surface proteins GspB and Hsa mediate binding to sialylated carbohydrate epitopes on the platelet membrane glycoprotein Ibalpha. Infect Immun. 2004; 72(11):6528-37. PMC: 523053. DOI: 10.1128/IAI.72.11.6528-6537.2004. View

Veerachamy S, Yarlagadda T, Manivasagam G, Yarlagadda P . Bacterial adherence and biofilm formation on medical implants: a review. Proc Inst Mech Eng H. 2014; 228(10):1083-99. DOI: 10.1177/0954411914556137. View

. NTP Toxicology and Carcinogenesis Studies of Polysorbate 80 (CAS No. 9005-65-6) in F344/N Rats and B6C3F1 Mice (Feed Studies). Natl Toxicol Program Tech Rep Ser. 1992; 415:1-225. View

Utsui Y, Yokota T . Role of an altered penicillin-binding protein in methicillin- and cephem-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 1985; 28(3):397-403. PMC: 180261. DOI: 10.1128/AAC.28.3.397. View

DeLeo F, Chambers H . Reemergence of antibiotic-resistant Staphylococcus aureus in the genomics era. J Clin Invest. 2009; 119(9):2464-74. PMC: 2735934. DOI: 10.1172/JCI38226. View

Kiedrowski M, Horswill A . New approaches for treating staphylococcal biofilm infections. Ann N Y Acad Sci. 2011; 1241:104-21. DOI: 10.1111/j.1749-6632.2011.06281.x. View

10.

Heilmann C . Adhesion mechanisms of staphylococci. Adv Exp Med Biol. 2011; 715:105-23. DOI: 10.1007/978-94-007-0940-9_7. View

11.

Makino T, Jimi S, Oyama T, Nakano Y, Hamamoto K, Mamishin K . Infection mechanism of biofilm-forming Staphylococcus aureus on indwelling foreign materials in mice. Int Wound J. 2013; 12(2):122-31. PMC: 7950666. DOI: 10.1111/iwj.12061. View

12.

Blomberg C, Dagerhamn J, Dahlberg S, Browall S, Fernebro J, Albiger B . Pattern of accessory regions and invasive disease potential in Streptococcus pneumoniae. J Infect Dis. 2009; 199(7):1032-42. DOI: 10.1086/597205. View

13.

Otto M . Staphylococcal infections: mechanisms of biofilm maturation and detachment as critical determinants of pathogenicity. Annu Rev Med. 2012; 64:175-88. DOI: 10.1146/annurev-med-042711-140023. View

14.

Scarselli M, Serruto D, Montanari P, Capecchi B, Adu-Bobie J, Veggi D . Neisseria meningitidis NhhA is a multifunctional trimeric autotransporter adhesin. Mol Microbiol. 2006; 61(3):631-44. DOI: 10.1111/j.1365-2958.2006.05261.x. View

15.

Sloup R, Cieza R, Needle D, Abramovitch R, Torres A, Waters C . Polysorbates prevent biofilm formation and pathogenesis of Escherichia coli O104:H4. Biofouling. 2016; 32(9):1131-1140. PMC: 5176131. DOI: 10.1080/08927014.2016.1230849. View

16.

Stewart P . Mechanisms of antibiotic resistance in bacterial biofilms. Int J Med Microbiol. 2002; 292(2):107-13. DOI: 10.1078/1438-4221-00196. View

17.

Schroeder K, Jularic M, Horsburgh S, Hirschhausen N, Neumann C, Bertling A . Molecular characterization of a novel Staphylococcus aureus surface protein (SasC) involved in cell aggregation and biofilm accumulation. PLoS One. 2009; 4(10):e7567. PMC: 2761602. DOI: 10.1371/journal.pone.0007567. View

18.

Roche F, Downer R, Keane F, Speziale P, Park P, Foster T . The N-terminal A domain of fibronectin-binding proteins A and B promotes adhesion of Staphylococcus aureus to elastin. J Biol Chem. 2004; 279(37):38433-40. DOI: 10.1074/jbc.M402122200. View

19.

Jimi S, Miyazaki M, Takata T, Ohjimi H, Akita S, Hara S . Increased drug resistance of meticillin-resistant Staphylococcus aureus biofilms formed on a mouse dermal chip model. J Med Microbiol. 2017; 66(4):542-550. PMC: 5817199. DOI: 10.1099/jmm.0.000461. View

20.

Clarke S, Foster S . Surface adhesins of Staphylococcus aureus. Adv Microb Physiol. 2006; 51:187-224. DOI: 10.1016/S0065-2911(06)51004-5. View