Genes for Bacillus Megaterium Small, Acid-soluble Spore Proteins: Cloning and Nucleotide Sequence of Three Additional Genes from This Multigene Family

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1986 Feb 1

PMID 3080406

Citations 11

Authors

E R Fliss

C A Loshon

P Setlow

Affiliations

Soon will be listed here.

Abstract

Three genes coding for small, acid-soluble spore proteins (SASP) were cloned from Bacillus megaterium, using previously cloned B. megaterium SASP genes (SASP-C and -C-3) as DNA-DNA hybridization probes. One gene (SASP-A) codes for the A protein, a previously identified major SASP. The other two (termed genes for SASP-C-4 and -C-5) are extremely similar in much of their nucleotide sequence to the previously cloned B. megaterium SASP-C-2 gene. The proteins coded for by all these SASP genes had extensive sequence homology with each other and with those coded for by the B. megaterium SASP-C, -C-1, -C-2, and -C-3 genes. Their coding sequences are preceded by strong ribosome-binding sites and are followed by regions of dyad symmetry which presumably are transcription stop sites. The SASP-A, -C-4, and -C-5 genes are expressed in parallel during sporulation, and their transcription start points were localized by the size of the mRNAs produced. The sequences localized 10 and 35 base pairs upstream from the transcription start points show significant homology with the analogous regions of the SASP-C, -C-1, -C-2, and -C-3 genes. The identification of seven closely related SASP genes in B. megaterium indicates that the SASP are the products of a very extensive multigene family.

Citing Articles

Genomic determinants of sporulation in Bacilli and Clostridia: towards the minimal set of sporulation-specific genes.

Galperin M, Mekhedov S, Puigbo P, Smirnov S, Wolf Y, Rigden D Environ Microbiol. 2012; 14(11):2870-90.

PMID: 22882546 PMC: 3533761. DOI: 10.1111/j.1462-2920.2012.02841.x.

Polyhydroxyalkanoate inclusion body-associated proteins and coding region in Bacillus megaterium.

McCool G, Cannon M J Bacteriol. 1999; 181(2):585-92.

PMID: 9882674 PMC: 93414. DOI: 10.1128/JB.181.2.585-592.1999.

Cloning and nucleotide sequence of a plasmid-carried gene coding for a minor small, acid-soluble protein from Bacillus megaterium spores.

Setlow P J Bacteriol. 1993; 175(19):6337-40.

PMID: 8407806 PMC: 206731. DOI: 10.1128/jb.175.19.6337-6340.1993.

Reiterated DNA sequences in Rhizobium and Agrobacterium spp.

Flores M, Gonzalez V, Brom S, Martinez E, Pinero D, Romero D J Bacteriol. 1987; 169(12):5782-8.

PMID: 3450286 PMC: 214138. DOI: 10.1128/jb.169.12.5782-5788.1987.

Integration and mapping of Bacillus megaterium genes which code for small, acid-soluble spore proteins and their protease.

Sussman M, Vary P, Hartman C, Setlow P J Bacteriol. 1988; 170(10):4942-5.

PMID: 3139641 PMC: 211542. DOI: 10.1128/jb.170.10.4942-4945.1988.

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