Detection of Ureaplasma Spp. Serovars in Genital Tract of Infertile Males

Overview

Journal J Clin Lab Anal

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
Pathology

Date 2019 Feb 26

PMID 30803037

Citations 4

Authors

Tiejun Song

Zhiwei Liu

Ying Zhang

Yetao Han

Jun Huang

Affiliations

Soon will be listed here.

Abstract

Background: The colonization of Ureaplasma species in genital tract is related with male infertility. However, it has been postulated based upon limited study that virulence is related to serotype specificity. The aim of this study was to determine the distribution of Ureaplasma serovars in genital tract of infertile males and analyze their role in male infertility.

Methods: A total of 358 urethral swabs samples were obtained from infertile males. The culture of Ureaplasma species were performed using a commercially available Mycoplasma IST 2 kit. Serovars were determined by real-time polymerase chain reaction (real-time PCR).

Results: A total of 92 (25.7%) infertile males were positive for Ureaplasma spp; among them, Ureaplasma parvum (UPA) was detected in 73 (79.3%) isolates, and Ureaplasma urealyticum (UUR) was detected in 19 (20.7%) isolates. Serovars 1, 6, or in combination accounted for 63.0% (46/73) of UPA isolates. Serovar 9 (alone and in combination of other serovars) was the most common serovar in UUR (47.4%, 9/19). Multiple serovars were detected in 21 (22.8%) isolates, and serovars 4, 5, 7, and 12 were not detected in any sample.

Conclusion: The distribution of 14 Ureaplasma serovars in genital tract of infertile males was identified for the first time by real-time PCR assay. UPA serovars 1 and 6, and UUR serovar 9 are the most common serovars colonization in urogenital tract of infertile males.

Citing Articles

An overview regarding the relationship between Mollicutes, infertility and antibiotic resistance (Review).

Cutoiu A, Boda D Biomed Rep. 2024; 21(2):119.

PMID: 38938741 PMC: 11209857. DOI: 10.3892/br.2024.1807.

Normospermic Patients Infected With Role of Dysregulated miR-122-5p, miR-34c-5, and miR-141-3p.

Galdiero M, Trotta C, Schettino M, Cirillo L, Sasso F, Petrillo F Pathog Immun. 2024; 8(2):16-36.

PMID: 38223489 PMC: 10783813. DOI: 10.20411/pai.v8i2.603.

Results from a large cross-sectional study assessing Chlamydia trachomatis, Ureaplasma spp. and Mycoplasma hominis urogenital infections in patients with primary infertility.

Paira D, Molina G, Tissera A, Olivera C, Molina R, Motrich R Sci Rep. 2021; 11(1):13655.

PMID: 34211075 PMC: 8249471. DOI: 10.1038/s41598-021-93318-1.

Detection of Ureaplasma spp. serovars in genital tract of infertile males.

Song T, Liu Z, Zhang Y, Han Y, Huang J J Clin Lab Anal. 2019; 33(5):e22865.

PMID: 30803037 PMC: 6595327. DOI: 10.1002/jcla.22865.

References

Xiao L, Glass J, Paralanov V, Yooseph S, Cassell G, Duffy L . Detection and characterization of human Ureaplasma species and serovars by real-time PCR. J Clin Microbiol. 2010; 48(8):2715-23. PMC: 2916572. DOI: 10.1128/JCM.01877-09. View

Reichart M, Levi H, Kahane I, Bartoov B . Dual energy metabolism-dependent effect of Ureaplasma urealyticum infection on sperm activity. J Androl. 2001; 22(3):404-12. View

Zdrodowska-Stefanow B, Klosowska W, Ostaszewska-Puchalska I, Bulhak-Koziol V, Kotowicz B . Ureaplasma urealyticum and Mycoplasma hominis infection in women with urogenital diseases. Adv Med Sci. 2007; 51:250-3. View

Cao X, Wang Y, Hu X, Qing H, Wang H . Real-time TaqMan polymerase chain reaction assays for quantitative detection and differentiation of Ureaplasma urealyticum and Ureaplasma parvum. Diagn Microbiol Infect Dis. 2006; 57(4):373-8. DOI: 10.1016/j.diagmicrobio.2006.09.006. View

Gump D, Gibson M, Ashikaga T . Lack of association between genital mycoplasmas and infertility. N Engl J Med. 1984; 310(15):937-41. DOI: 10.1056/NEJM198404123101501. View

Gdoura R, Kchaou W, Chaari C, Znazen A, Keskes L, Rebai T . Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis and Mycoplasma genitalium infections and semen quality of infertile men. BMC Infect Dis. 2007; 7:129. PMC: 2194714. DOI: 10.1186/1471-2334-7-129. View

Xia X, An L, Li W, Li K, Shao Y, Shang X . [Ureaplasma urealyticum infection affects sperm plasma membrane integrity in infertile men]. Zhonghua Nan Ke Xue. 2012; 17(12):1069-72. View

Song T, Liu Z, Zhang Y, Han Y, Huang J . Detection of Ureaplasma spp. serovars in genital tract of infertile males. J Clin Lab Anal. 2019; 33(5):e22865. PMC: 6595327. DOI: 10.1002/jcla.22865. View

Kong F, Ma Z, James G, Gordon S, Gilbert G . Species identification and subtyping of Ureaplasma parvum and Ureaplasma urealyticum using PCR-based assays. J Clin Microbiol. 2000; 38(3):1175-9. PMC: 86368. DOI: 10.1128/JCM.38.3.1175-1179.2000. View

10.

Paralanov V, Lu J, Duffy L, Crabb D, Shrivastava S, Methe B . Comparative genome analysis of 19 Ureaplasma urealyticum and Ureaplasma parvum strains. BMC Microbiol. 2012; 12:88. PMC: 3511179. DOI: 10.1186/1471-2180-12-88. View

11.

Kanakas N, Mantzavinos T, Boufidou F, Koumentakou I, Creatsas G . Ureaplasma urealyticum in semen: is there any effect on in vitro fertilization outcome?. Fertil Steril. 1999; 71(3):523-7. DOI: 10.1016/s0015-0282(98)00472-5. View

12.

Abusarah E, Awwad Z, Charvalos E, Shehabi A . Molecular detection of potential sexually transmitted pathogens in semen and urine specimens of infertile and fertile males. Diagn Microbiol Infect Dis. 2013; 77(4):283-6. DOI: 10.1016/j.diagmicrobio.2013.05.018. View

13.

Xiao L, Paralanov V, Glass J, Duffy L, Robertson J, Cassell G . Extensive horizontal gene transfer in ureaplasmas from humans questions the utility of serotyping for diagnostic purposes. J Clin Microbiol. 2011; 49(8):2818-26. PMC: 3147716. DOI: 10.1128/JCM.00637-11. View

14.

Salmeri M, Valenti D, La Vignera S, Bellanca S, Morello A, Toscano M . Prevalence of Ureaplasma urealyticum and Mycoplasma hominis infection in unselected infertile men. J Chemother. 2012; 24(2):81-6. DOI: 10.1179/1120009X12Z.00000000021. View

15.

Xu C, Sun G, Zhu Y, Wang Y . The correlation of Ureaplasma urealyticum infection with infertility. Andrologia. 1997; 29(4):219-26. DOI: 10.1111/j.1439-0272.1997.tb00319.x. View

16.

Zhang J, Kong Y, Feng Y, Huang J, Song T, Ruan Z . Development of a multilocus sequence typing scheme for Ureaplasma. Eur J Clin Microbiol Infect Dis. 2013; 33(4):537-44. DOI: 10.1007/s10096-013-1981-y. View

17.

Song T, Ye A, Xie X, Huang J, Ruan Z, Kong Y . Epidemiological investigation and antimicrobial susceptibility analysis of ureaplasma species and Mycoplasma hominis in outpatients with genital manifestations. J Clin Pathol. 2014; 67(9):817-20. DOI: 10.1136/jclinpath-2014-202248. View

18.

Potts J, Sharma R, Pasqualotto F, Nelson D, Hall G, Agarwal A . Association of ureaplasma urealyticum with abnormal reactive oxygen species levels and absence of leukocytospermia. J Urol. 2000; 163(6):1775-8. View

19.

Zhang J, Kong Y, Ruan Z, Huang J, Song T, Song J . Correlation between Ureaplasma subgroup 2 and genitourinary tract disease outcomes revealed by an expanded multilocus sequence typing (eMLST) scheme. PLoS One. 2014; 9(8):e104347. PMC: 4122457. DOI: 10.1371/journal.pone.0104347. View

20.

Waites K, Katz B, Schelonka R . Mycoplasmas and ureaplasmas as neonatal pathogens. Clin Microbiol Rev. 2005; 18(4):757-89. PMC: 1265909. DOI: 10.1128/CMR.18.4.757-789.2005. View