The Effect of Microfluidic Geometry on Myoblast Migration

Overview

Journal Micromachines (Basel)

Publisher MDPI

Date 2019 Feb 24

PMID 30795574

Citations 2

Authors

Rahul Atmaramani

Bryan J Black

Kevin H Lam

Vinit M Sheth

Joseph J Pancrazio

David W Schmidtke

Nesreen Zoghoul Alsmadi

Affiliations

Soon will be listed here.

Abstract

In vitro systems comprised of wells interconnected by microchannels have emerged as a platform for the study of cell migration or multicellular models. In the present study, we systematically evaluated the effect of microchannel width on spontaneous myoblast migration across these microchannels-from the proximal to the distal chamber. Myoblast migration was examined in microfluidic devices with varying microchannel widths of 1.5⁻20 µm, and in chips with uniform microchannel widths over time spans that are relevant for myoblast-to-myofiber differentiation in vitro. We found that the likelihood of spontaneous myoblast migration was microchannel width dependent and that a width of 3 µm was necessary to limit spontaneous migration below 5% of cells in the seeded well after 48 h. These results inform the future design of Polydimethylsiloxane (PDMS) microchannel-based co-culture platforms as well as future in vitro studies of myoblast migration.

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